製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to hCAP-D3
  • 特異性
    We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide corresponding to a region between C terminal residues 1450-1507 of human hCAP-D3. (NP_056076.1)

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70349 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報
    IP: Use at 1 to 4 µg/mg of lysate
    WB: 1/2000 - 1/10000. Detects a band of approximately 174 kDa (predicted molecular weight: 168 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis.
    • 配列類似性
      Contains 4 HEAT repeats.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CAP D3 antibody
      • CAPD 3 antibody
      • CAPD3 antibody
      • CNDD3_HUMAN antibody
      • Condensin II complex subunit D3 antibody
      • Condensin-2 complex subunit D3 antibody
      • FLJ42888 antibody
      • hCAP-D3 antibody
      • Hcp 6 antibody
      • Hcp6 antibody
      • hHCP 6 antibody
      • hHCP6 antibody
      • KIAA0056 antibody
      • MGC104671 antibody
      • NCAPD 3 antibody
      • Ncapd3 antibody
      • Non SMC condensin II complex subunit D3 antibody
      • Non-SMC condensin II complex subunit D3 antibody
      • OTTHUMP00000235403 antibody
      • OTTHUMP00000235404 antibody
      see all

    画像

    • All lanes:200µg Hela whole cell lysate and ab70349 at 1µg/ml. Lane 1: ab70349 at 3 µg/mg lysate for IP. Lane 2: IgG control.
    • All lanes : Anti-hCAP-D3 antibody (ab70349) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 168 kDa
      Observed band size : 174 kDa (why is the actual band size different from the predicted?)

    参考文献

    This product has been referenced in:

    See all 3 Publications for this product

    レビューと Q&A

    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (fibroblast)
    Loading amount
    50 µg
    Specification
    fibroblast
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 May 10 2013

    Thank you for contacting us.
    As discussed please let me know if you are interested in the 100% Abreview testing discount programme.
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