製品の概要
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製品名Anti-HBP1 antibody
HBP1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to HBP1
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由来種Rabbit
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アプリケーション適用あり: WB, ELISAmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
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免疫原
Synthetic peptide corresponding to a region within internal sequence amino acids 465-514 (SVILGDRWKK MKNEERRMYT LEAKALAEEQ KRLNPDCWKR KRTNSGSQQH) of Human HBP1 (NP_036389).
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ポジティブ･コントロール
- HeLa cell lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab83402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| ELISA | Use at an assay dependent concentration. ELISA titre using peptide based assay: 1/1562500. |
ターゲット情報
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機能Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4.
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配列類似性Contains 1 AXH domain.
Contains 1 HMG box DNA-binding domain. -
細胞内局在Nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 417705 Chicken
- Entrez Gene: 515320 Cow
- Entrez Gene: 475886 Dog
- Entrez Gene: 26959 Human
- Entrez Gene: 73389 Mouse
- Entrez Gene: 27080 Rat
- Omim: 616714 Human
- SwissProt: Q2KJ34 Cow
see all -
別名
- FLJ16340 antibody
- HBP 1 antibody
- HBP1 antibody
see all
画像
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Western blot - HBP1 antibody (ab83402)Anti-HBP1 antibody (ab83402) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
Gel concentration: 12%
プロトコール
参考文献
This product has been referenced in:
- Terashima M et al. Functional Analyses of Mutations in Receptor Tyrosine Kinase Genes in Non-Small Cell Lung Cancer: Double-Edged Sword of DDR2. Clin Cancer Res 22:3663-71 (2016). Read more (PubMed: 26826182) »
- Yang Z et al. MiR-29a modulates the angiogenic properties of human endothelial cells. Biochem Biophys Res Commun 434:143-9 (2013). Read more (PubMed: 23541945) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"