製品の概要

  • 製品名Anti-HB9/HLXB9 antibody [EPR3342]
    HB9/HLXB9 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3342] to HB9/HLXB9
  • 特異性There have been conflicting results with this antibody in IHC-P. PMID 21484430 reported specific labeling in IHC-P, but internally we were unable to reproduce these results. Thus, we have removed IHC-P as a guaranteed application, and we welcome any further feedback from customers using this antibody in IHC-P.
  • アプリケーション適用あり: WB, IPmore details
    適用なし: Flow Cyt or ICC
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C terminal of Human HB9/HLXB9.

  • ポジティブ・コントロール
    • Molt-4 and Raji cell lysates.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab79541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa).
IP 1/50.
  • 追加情報Is unsuitable for Flow Cyt or ICC.
  • ターゲット情報

    • 機能Putative transcription factor involved in pancreas development and function.
    • 組織特異性Expressed in lymphoid and pancreatic tissues.
    • 関連疾患Defects in MNX1 are a cause of Currarino syndrome (CURRAS) [MIM:176450]. The triad of a presacral tumor, sacral agenesis and anorectal malformation constitutes the Currarino syndrome which is caused by dorsal-ventral patterning defects during embryonic development. The syndrome occurs in the majority of patients as an autosomal dominant trait.
    • 配列類似性Contains 1 homeobox DNA-binding domain.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • HB 9 antibody
      • HB9 antibody
      • HLXB 9 antibody
      • HLXB9 antibody
      • Homeo box HB9 antibody
      • Homeobox HB9 antibody
      • Homeobox protein HB9 antibody
      • HOXHB9 antibody
      • MNX1 antibody
      • MNX1_HUMAN antibody
      • Motor neuron and pancreas homeobox protein 1 antibody
      • Sacral agenesis autosomal dominant (Currarino triad) antibody
      • SCRA 1 antibody
      • SCRA1 antibody
      see all

    Anti-HB9/HLXB9 antibody [EPR3342] 画像

    • All lanes : Anti-HB9/HLXB9 antibody [EPR3342] (ab79541) at 1/200000 dilution

      Lane 1 : Molt-4 cell lysate
      Lane 2 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 41 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)

    Anti-HB9/HLXB9 antibody [EPR3342] (ab79541) 使用論文

    This product has been referenced in:
    • Egawa N  et al. Drug screening for ALS using patient-specific induced pluripotent stem cells. Sci Transl Med 4:145ra104 (2012). Human . Read more (PubMed: 22855461) »
    • Wilkens L  et al. The homeobox gene HLXB9 is upregulated in a morphological subset of poorly differentiated hepatocellular carcinoma. Virchows Arch 458:697-708 (2011). WB, IHC-P ; Human . Read more (PubMed: 21484430) »

    See all 2 Publications for this product

    Product Wall

    I will send the IHC protocol for ab59795 when I receive it, hoepfully in the next day or two. As I mentioned, Abcam does not validate this antibody for IHC in-house. We do not have free samples of any of our antibodies, but ab59795 is covered by our Ab...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"