Anti-HB9/HLXB9/MNX1 抗体 [EPR3342] (ab79541)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3342] to HB9/HLXB9/MNX1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-HB9/HLXB9/MNX1 antibody [EPR3342]
HB9/HLXB9/MNX1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3342] to HB9/HLXB9/MNX1 -
由来種
Rabbit -
特異性
There have been conflicting results with this antibody in IHC-P. PMID 21484430 reported specific labeling in IHC-P, but internally we were unable to reproduce these results. Thus, we have removed IHC-P as a guaranteed application, and we welcome any further feedback from customers using this antibody in IHC-P. -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide within Human HB9/HLXB9/MNX1 aa 250-350 (C terminal). The exact sequence is proprietary.
-
ポジティブ・コントロール
- Molt-4 and Raji cell lysates.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3342 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79541の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa).
|
特記事項 |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa). |
ターゲット情報
-
機能
Putative transcription factor involved in pancreas development and function. -
組織特異性
Expressed in lymphoid and pancreatic tissues. -
関連疾患
Defects in MNX1 are a cause of Currarino syndrome (CURRAS) [MIM:176450]. The triad of a presacral tumor, sacral agenesis and anorectal malformation constitutes the Currarino syndrome which is caused by dorsal-ventral patterning defects during embryonic development. The syndrome occurs in the majority of patients as an autosomal dominant trait. -
配列類似性
Contains 1 homeobox DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 3110 Human
- Entrez Gene: 15285 Mouse
- Omim: 142994 Human
- SwissProt: P50219 Human
- SwissProt: Q9QZW9 Mouse
- Unigene: 37035 Human
- Unigene: 103760 Mouse
-
別名
- HB 9 antibody
- HB9 antibody
- HLXB 9 antibody
see all
画像
-
All lanes : Anti-HB9/HLXB9/MNX1 antibody [EPR3342] (ab79541) at 1/200000 dilution
Lane 1 : Molt-4 cell lysate
Lane 2 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (6)
ab79541 は 6 報の論文で使用されています。
- Zhu B et al. MNX1 Promotes Malignant Progression of Cervical Cancer via Repressing the Transcription of p21cip1. Front Oncol 10:1307 (2020). PubMed: 32850410
- Park S et al. Differentiation of Motor Neuron-Like Cells from Tonsil-Derived Mesenchymal Stem Cells and Their Possible Application to Neuromuscular Junction Formation. Int J Mol Sci 20:N/A (2019). PubMed: 31159418
- Egawa N et al. Drug screening for ALS using patient-specific induced pluripotent stem cells. Sci Transl Med 4:145ra104 (2012). Human . PubMed: 22855461
- Ruggiu M et al. A role for SMN exon 7 splicing in the selective vulnerability of motor neurons in spinal muscular atrophy. Mol Cell Biol 32:126-38 (2012). PubMed: 22037760
- Wu CY et al. Proteomic assessment of a cell model of spinal muscular atrophy. BMC Neurosci 12:25 (2011). PubMed: 21385431
- Wilkens L et al. The homeobox gene HLXB9 is upregulated in a morphological subset of poorly differentiated hepatocellular carcinoma. Virchows Arch 458:697-708 (2011). IHC-P, WB ; Human . PubMed: 21484430