製品の概要

  • 製品名Anti-HAUS5 antibody
  • 製品の詳細
    Rabbit polyclonal to HAUS5
  • アプリケーション適用あり: Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human HAUS5 aa 139-167 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • ポジティブ・コントロール
    • Human colon carcinoma.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)This antibody is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab103721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性HAUS5 is 1 of 8 subunits of the 390-kD Human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly.
  • 細胞内局在Cytoplasm, cytoskeleton, centrosome and spindle.
  • 参照データベース
  • 別名
    • Dgt5 antibody
    • HAUS augmin like complex subunit 5 antibody
    • HAUS augmin like complex, subunit 5 antibody
    • KIAA0841 antibody

Anti-HAUS5 antibody 画像

  • HAUS5 Antibody (N-term) (ab103721) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • ab103721 at 1/50 dilution staining HAUS5 by immunohistochemistry in formalin fixed and paraffin embedded Human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Anti-HAUS5 antibody (ab103721) 使用論文

ab103721 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Xenopus laevis Cell (Spindles in X. laevis cytoplasmic egg extract)
Specification Spindles in X. laevis cytoplasmic egg extract
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Oct 05 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Cell lysate - other (Xenopus laevis cytoplasmic egg extract)
Loading amount 50 µg
Specification Xenopus laevis cytoplasmic egg extract
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Oct 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"