製品の概要

  • 製品名
    Anti-HAUS4 antibody
  • 製品の詳細
    Rabbit polyclonal to HAUS4
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 296-326 of Human HAUS4.

  • ポジティブ・コントロール
    • MCF7 cell line lysates. Skin carcinoma.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab103709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 42 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性
    This gene encodes a subunit of the centrosome complex termed the human augmin complex. The encoded protein localizes to the spindle microtubules and may play a role in mitotic spindle assembly and maintenance of centrosome integrity during cell division. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 1.
  • 細胞内局在
    Cytoplasm › cytoskeleton › centrosome. Cytoplasm › cytoskeleton › spindle.
  • 参照データベース
  • 別名
    • C14orf94 antibody
    • Chromosome 14 open reading frame 94 antibody
    • FLJ20424 antibody
    • FLJ40661 antibody
    • HAUS augmin like complex subunit 4 antibody
    • Homologous to Augmin subunits antibody
    • OTTHUMP00000164554 antibody
    see all

画像

  • Anti-HAUS4 antibody (ab103709) at 1/100 dilution + MCF7 cell line lysates at 35 µg

    Predicted band size : 42 kDa
  • ab103709 at 1/50 dilution staining HAUS4 in formalin-fixed and paraffin-embedded skin carcinoma by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Detection utilised DAB.

参考文献

ab103709 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus laevis Cell (Spindles in X. laevis cytoplasmic egg extract)
Specification
Spindles in X. laevis cytoplasmic egg extract
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Oct 05 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Xenopus laevis Cell lysate - other (Xenopus laevis cytoplasmic egg extract)
Loading amount
50 µg
Specification
Xenopus laevis cytoplasmic egg extract
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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