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Human haptoglobin purified from serum. All Hp types (Hp1-1, Hp1-2 and Hp2-2) are present in the immunogen.
Our Abpromise guarantee covers the use of ab8968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Double Immunodiffusion||Use at an assay dependent dilution. 10µL antiserum vs 5µL serum|
|RID||Use at an assay dependent dilution. (and Rocket IEP) 2µL antiserum/cm² gel vs 5µL neat – 1/10 dilution human serum.
The use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. Suitability for use in nephelometry, immunohistochemical procedures, enzyme-linked immunosorbent assays and Western blot has not been assessed but use in such assays should not necessarily be excluded.
|Immunoelectrophoresis||Use at an assay dependent dilution. 100µL antiserum vs 5µL serum|
|Rocket Immunoelectrophoresis||Use at an assay dependent concentration.|