製品の概要

  • 製品名Anti-Haptoglobin antibody
    Haptoglobin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Haptoglobin
  • アプリケーション適用あり: WB, ELISA, IHC-P, Sandwich ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human Haptoglobin

  • ポジティブ・コントロール
    • Human liver tissue

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)ab85846 recognizes Human Haptoglobin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab85846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 45 kDa.
ELISA 1/3000 - 1/12000.
IHC-P Use a concentration of 2.5 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse polyclonal to Haptoglobin (ab52652) as Capture.

ターゲット情報

  • 機能As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
    Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
  • 組織特異性Expressed by the liver and secreted in plasma.
  • 関連疾患Anhaptoglobinemia
  • 配列類似性Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Binding peptide antibody
    • BP antibody
    • Haptoglobin alpha chain antibody
    • Haptoglobin alpha(1S) beta antibody
    • Haptoglobin alpha(2FS) beta antibody
    • Haptoglobin beta chain antibody
    • Haptoglobin, alpha polypeptide antibody
    • Haptoglobin, beta polypeptide antibody
    • HP antibody
    • HP2 ALPHA2 antibody
    • HP2ALPHA2 antibody
    • HPA1S antibody
    • HPT antibody
    • HPT_HUMAN antibody
    • MGC111141 antibody
    • Zonulin antibody
    see all

Anti-Haptoglobin antibody 画像

プロトコール

Anti-Haptoglobin antibody (ab85846) 使用論文

This product has been referenced in:
  • Tan Y  et al. Acute coronary syndrome remodels the protein cargo and functions of high-density lipoprotein subfractions. PLoS One 9:e94264 (2014). WB ; Human . Read more (PubMed: 24736723) »

See 1 Publication for this product

Product Wall

These two products will be suitable standards:

Hemoglobin - ab77858 (purified from Human erythrocytes)
Haptoglobin - ab77872 (purified from Human plasma)

Please contact us if you have additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"