Anti-Haploid sperm cell-specific antigen 抗体 [TRA54] (ab92286)

製品の概要

  • 製品名
    Anti-Haploid sperm cell-specific antigen antibody [TRA54]
  • 製品の詳細
    Rat monoclonal [TRA54] to Haploid sperm cell-specific antigen
  • アプリケーション
    適用あり: WB, Electron Microscopy, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Adult Mouse testis.

  • ポジティブ・コントロール
    • Mouse testis lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: None
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • 特記事項(精製)
    ab92286 was purified from the serum-free culture medium of the hybridoma clone TRA54 by proprietary chromatography processes under mild conditions.
  • ポリ/モノ
    モノクローナル
  • クローン名
    TRA54
  • アイソタイプ
    IgM
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab92286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml.
Electron Microscopy Use at an assay dependent dilution. PubMed: 12857863
IHC-P Use at an assay dependent dilution.

ターゲット情報

  • 関連性
    Many transcripts for proteins specifically expressed in haploid germ cells are synthesized in the spermatogenesis. A monoclonal antibody TRA54 recognizes specific organellaes in germ cell cytoplasm from spermatocytes to spermatids.

画像

  • All lanes : Anti-Haploid sperm cell-specific antigen antibody [TRA54] (ab92286) at 1/5000 dilution

    Lane 1 : Mouse brain cell lysate
    Lane 2 : Mouse Heart cell lysate
    Lane 3 : Mouse Intestine cell lysate
    Lane 4 : Mouse Kidney cell lysate
    Lane 5 : Mouse Liver cell lysate
    Lane 6 : Mouse Muscle cell lysate
    Lane 7 : Mouse Ovary cell lysate
    Lane 8 : Mouse Skin cell lysate
    Lane 9 : Mouse Spleen cell lysate
    Lane 10 : Mouse Testis cell lysate

    Lysates/proteins at 100 µg per lane.


    Observed band size : 190,85,>200 kDa (why is the actual band size different from the predicted?)

参考文献

This product has been referenced in:
  • Arroteia KF  et al. Albumin is synthesized in epididymis and aggregates in a high molecular mass glycoprotein complex involved in sperm-egg fertilization. PLoS One 9:e103566 (2014). WB ; Mouse . Read more (PubMed: 25084016) »
  • Arrotéia KF  et al. Identification and characterization of an antigen recognized by monoclonal antibody TRA 54 in mouse epididymis and vas deferens. J Androl 25:914-21 (2004). Read more (PubMed: 15477363) »

See all 4 Publications for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab92286.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録