This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

製品の概要

製品の特性

アプリケーション

Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use

アプリケーション Abreviews 特記事項
ICC/IF 1/150. This Fast-track antibody has been successfully used for ICC/IF using rat oligodendrocyte cell culture. However, please be aware that this information should be used for information purposes only, and that Abcam cannot guarantee that all batches of this antibody will be equally suitable.
WB Use a concentration of 5 µg/ml. This Fast-track antibody has been successfully used for WB using rat brain tissue lysates. However, please be aware that this information should be used for information purposes only, and that Abcam cannot guarantee that all batches of this antibody will be equally suitable.
ELISA Use at an assay dependent concentration. : This antibody gave a positive result in ELISA against the immunizing peptide (ab37539).
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. This Fast-track antibody has been successfully used for IHC-P on oral mucosa tissue. However, please be aware that this information should be used for information purposes only, and that Abcam cannot guarantee that all batches of this antibody will be equally suitable.

ターゲット情報

  • 機能
    May act as a transcription factor that could act on to regulate one of the phases of hair growth.
  • 組織特異性
    Strongest expression of isoforms 1 and 2 is seen in the small intestine, weaker expression in brain and colon, and trace expression is found in liver, pancreas, spleen, thymus, stomach, salivary gland, appendix and trachea. Isoform 1 is always the most abundant. Isoform 1 is exclusively expressed at low levels in kidney and testis. Isoform 2 is exclusively expressed at high levels in the skin.
  • 関連疾患
    Defects in HR are the cause of alopecia universalis congenita (ALUNC) [MIM:203655]. ALUNC is a rare autosomal recessive form of hair loss characterized by hair follicles without hair.
    Defects in HR are the cause of atrichia with papular lesions (APL) [MIM:209500]; also known as congenital atrichia. APL is an autosomal recessive disease characterized by papillary lesions over most of the body and almost complete absence of hair.
    Defects in HR are the cause of hypotrichosis Marie Unna type 1 (MUHH1) [MIM:146550]. An autosomal dominant condition characterized by reduced amount of hair, alopecia, little or no eyebrows, eyelashes or body hair, and coarse, wiry, twisted hair in early childhood.
  • 配列類似性
    Contains 1 JmjC domain.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ALUNC antibody
    • AU antibody
    • HAIR_HUMAN antibody
    • Hairless protein antibody
    • Host range antibody
    • HR antibody
    • HSA277165 antibody
    • Protein hairless antibody
    see all

参考文献

ab37540 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Rat Tissue lysate - whole (Brain)
Loading amount
30 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (12% Novex Gels)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Oct 11 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Oligodendrocyte)
Specification
Oligodendrocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% triton X-100
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Jul 08 2011

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Oral mucosa)
Specification
Oral mucosa
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric buffer, pH6
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
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投稿 Jun 07 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (P19)
Specification
P19
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% Triton X 100
Blocking step
Serum as blocking agent for 2 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 26°C
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投稿 Mar 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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