Anti-hair cortex Cytokeratin 抗体 [AE13] (ab16113)

製品の概要

  • 製品名
    Anti-hair cortex Cytokeratin antibody [AE13]
    hair cortex Cytokeratin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AE13] to hair cortex Cytokeratin
  • 特異性
    This antibody is specific for the family of hair cortex keratins.
  • アプリケーション
    適用あり: IHC-P, WB, IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Human hair keratins.

  • 特記事項


    This antibody is an excellent marker for hair and nail differentiation.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 一次抗体 備考
    This antibody is an excellent marker for hair and nail differentiation.
  • ポリ/モノ
    モノクローナル
  • クローン名
    AE13
  • ミエローマ
    P3x63-Ag8.653
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab16113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB 1/1000 - 1/3000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 - 46 kDa).
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/20 - 1/50. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    May play a role in late hair differentiation.
  • 組織特異性
    Expressed in skin and scalp. Also very weakly expressed in tongue, breast, colon and small intestine. In the hair follicle, it is specifically present in the upper hair cuticle. Not present in the upper cortex (at protein level).
  • 配列類似性
    Belongs to the intermediate filament family.
  • 発生段階
    During differentiation of the hair, it is one of the last keratins expressed.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CK-40 antibody
    • Cytokeratin-40 antibody
    • K1C40_HUMAN antibody
    • K40 antibody
    • Keratin antibody
    • Keratin, type I cytoskeletal 40 antibody
    • Keratin-40 antibody
    • Krt40 antibody
    • type I cytoskeletal 40 antibody
    • Type I hair keratin Ka36 antibody
    see all

Anti-hair cortex Cytokeratin antibody [AE13] 画像

  • ab16113 at 1/100 staining mouse skin tissue sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with BSA. A heat mediated antogen retrieval step was performed. The tissue was incubated with the antibody for 16 hours and then an Alexa-Fluor 488 conjugated goat anti-mouse antibody was used as the secondary. The image shows hair cortex cytokeratin staining in green with DAPI nuclei counterstain in blue.

    See Abreview

  • ab16113 staining hair cortex Cytokeratin in adult mouse nail by Immunohistochemistry (paraffin embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C followed by incubation with the primary antibody, at a 1/200 dilution, for 12 hours at 4°C. A Biotin-conjugated goat anti-mouse monoclonal was used as secondary antibody at a 1/400 dilution.

    See Abreview

  • All lanes : Anti-hair cortex Cytokeratin antibody [AE13] (ab16113) at 1 µg/ml

    Lane 1 : Mouse skin tissue lysate (14 days) (ab7271)
    Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 - 46 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 25 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • Overlay histogram showing A431 cells stained with ab16113 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16113, 1/20 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

Anti-hair cortex Cytokeratin antibody [AE13] (ab16113) 使用論文

This product has been referenced in:
  • Yuan S  et al. Post-transcriptional Regulation of Keratinocyte Progenitor Cell Expansion, Differentiation and Hair Follicle Regression by miR-22. PLoS Genet 11:e1005253 (2015). Read more (PubMed: 26020521) »
  • Wang D  et al. MicroRNA-205 controls neonatal expansion of skin stem cells by modulating the PI(3)K pathway. Nat Cell Biol 15:1153-63 (2013). Read more (PubMed: 23974039) »

See all 9 Publications for this product

Product Wall

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Skin)
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Ahmar Aziz

Verified customer

投稿 Jul 08 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Specification
skin
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: H-3300 Vector lab
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 01 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Adult nail)
Specification
Adult nail
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Mar 25 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Username

Abcam user community

Verified customer

投稿 Mar 09 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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