Anti-hair cortex Cytokeratin 抗体 (ab112444)

製品の概要

  • 製品名Anti-hair cortex Cytokeratin antibody
    hair cortex Cytokeratin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to hair cortex Cytokeratin
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human hair cortex Cytokeratin aa 1-100 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q6A162

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Human whole cell lysates: Caco2; MCF7; SW480; LOVO. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal skin.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab112444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

ターゲット情報

  • 機能May play a role in late hair differentiation.
  • 組織特異性Expressed in skin and scalp. Also very weakly expressed in tongue, breast, colon and small intestine. In the hair follicle, it is specifically present in the upper hair cuticle. Not present in the upper cortex (at protein level).
  • 配列類似性Belongs to the intermediate filament family.
  • 発生段階During differentiation of the hair, it is one of the last keratins expressed.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CK-40 antibody
    • Cytokeratin-40 antibody
    • K1C40_HUMAN antibody
    • K40 antibody
    • Keratin antibody
    • Keratin, type I cytoskeletal 40 antibody
    • Keratin-40 antibody
    • Krt40 antibody
    • type I cytoskeletal 40 antibody
    • Type I hair keratin Ka36 antibody
    see all

Anti-hair cortex Cytokeratin antibody 画像

  • IHC image of hair cortex Cytokeratin staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112444, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • All lanes : Anti-hair cortex Cytokeratin antibody (ab112444) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
    Lane 4 : LOVO (Human colon adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 48 kDa


    Exposure time : 2 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab112444 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Anti-hair cortex Cytokeratin antibody (ab112444) 使用論文

ab112444 has not yet been referenced specifically in any publications.

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