Anti-HADHSC 抗体 [1A12BC8] (ab110284)


  • 製品名
    Anti-HADHSC antibody [1A12BC8]
    HADHSC 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1A12BC8] to HADHSC
  • アプリケーション
    適用あり: IHC-P, ICC/IF, IP, Flow Cyt, In-Cell ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human liver mitochondria

  • ポジティブ・コントロール
    • MRC5 fibroblasts; Human cerebellum tissue; Human liver mitochondria; HL-60 cells.
  • 特記事項

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.



Our Abpromise guarantee covers the use of ab110284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/250. Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8.
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
In-Cell ELISA Use a concentration of 2 µg/ml. (0.2 µg/well).


  • 機能
    Plays an essential role in the mitochondrial beta-oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA.
  • 組織特異性
    Expressed in liver, kidney, pancreas, heart and skeletal muscle.
  • パスウェイ
    Lipid metabolism; fatty acid beta-oxidation.
  • 関連疾患
    Defects in HADH are the cause of 3-alpha-hydroxyacyl-CoA dehydrogenase deficiency (HADH deficiency) [MIM:231530]. HADH deficiency is a metabolic disorder with various clinical presentations including hypoglycemia, hepatoencephalopathy, myopathy or cardiomyopathy, and in some cases sudden death.
    Defects in HADH are the cause of familial hyperinsulinemic hypoglycemia type 4 (HHF4) [MIM:609975]; also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. It causes nesidioblastosis, a diffuse abnormality of the pancreas in which there is extensive, often disorganized formation of new islets. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur. HHF4 should be easily recognizable by analysis of acylcarnitine species and that this disorder responds well to treatment with diazoxide. It provides the first 'experiment of nature' that links impaired fatty acid oxidation to hyperinsulinism and that provides support for the concept that a lipid signaling pathway is implicated in the control of insulin secretion.
  • 配列類似性
    Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
  • 細胞内局在
    Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3 hydroxyacyl Coenzyme A dehydrogenase antibody
    • HAD antibody
    • HADH antibody
    • HADH1 antibody
    • HADHSC antibody
    • HADHSC, formerly antibody
    • HADSC, formerly antibody
    • HCDH antibody
    • HCDH_HUMAN antibody
    • HHF4 antibody
    • Hydroxyacyl CoA dehydrogenase antibody
    • Hydroxyacyl-coenzyme A dehydrogenase antibody
    • hydroxyacyl-coenzyme A dehydrogenase, mitochondrial antibody
    • L 3 hydroxyacyl Coenzyme A dehydrogenase short chain antibody
    • M SCHAD antibody
    • Medium and short chain L 3 hydroxyacyl coenzyme A dehydrogenase antibody
    • Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase antibody
    • MGC8392 antibody
    • mitochondrial antibody
    • MSCHAD antibody
    • OTTHUMP00000162626 antibody
    • OTTHUMP00000219688 antibody
    • SCHAD antibody
    • SCHAD, formerly antibody
    • Short chain 3 hydroxyacyl CoA dehydrogenase mitochondrial antibody
    • short chain 3-hydroxyacyl-coa dehydrogenase antibody
    • Short-chain 3-hydroxyacyl-CoA dehydrogenase antibody
    see all


  • MRC5 fibroblasts labeled with ab110284 at 5µg/ml and stained with Texas Red conjugated to goat anti-mouse secondary.
  • HADHSC immunocaptured from Human liver mitochondria using ab110284.
  • HL-60 cells were stained with 1 µg/ml ab110284 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • ab110284, at 1/250 dilution, staining HADHSC in formalin-fixed, paraffin-embedded Human cerebellum by Immunohistochemistry. HADHSC immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.


ab110284 has not yet been referenced specifically in any publications.

レビューと Q&A

DISCOUNT CODE: ********* Expiration date: *******I am very pleased to hear you would like to accept our offer and test ab110284 in mouse IP. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit...

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Thank you very much for your interest in ab110284. Below are the details of how the testing discount works. If you would still like to take advantage of the discount program, please let me know and I will issue the code: To our knowledge, ab110284 has ...

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Thank you for contacting Abcam. The epitope for ab110284, the anti-HADHSC antibody has not been mapped and so unfortunately I cannot tell you where it will bind on the protein. The antibody has not been tested in mouse, but I did an alignment between t...

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