Anti-HADHA/HADHB 抗体 [4A8BG12] (ab110302)

製品の概要

  • 製品名Anti-HADHA/HADHB antibody [4A8BG12]
  • 製品の詳細
    Mouse monoclonal [4A8BG12] to HADHA/HADHB
  • アプリケーション適用あり: ICC, IHC-P, Flow Cyt, IPmore details
  • 種交差性
    交差種: Rat, Cow, Human
  • 免疫原

    Human heart mitochondria

  • ポジティブ・コントロール
    • HDFn and HL60 cells; HepG2 cell lysate; Human cerebellum tissue.
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration. 0.5-2mg protein/10ul antibody

ターゲット情報

  • パスウェイLipid metabolism; fatty acid beta-oxidation.
  • 関連疾患Defects in HADHB are a cause of trifunctional protein deficiency (TFP deficiency) [MIM:609015]. The clinical manifestations are very variable and include hypoglycemia, cardiomyopathy and sudden death. Phenotypes with mainly hepatic and neuromyopathic involvement can also be distinguished. Biochemically, TFP deficiency is defined by the loss of all three enzyme activities of the TFP complex.
  • 配列類似性Belongs to the thiolase family.
  • 細胞内局在Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3-ketoacyl-CoA thiolase antibody
    • 4930479F15Rik antibody
    • Acetyl-CoA acyltransferase antibody
    • Beta-ketothiolase antibody
    • C77020 antibody
    • ECHA antibody
    • ECHB antibody
    • ECHB_HUMAN antibody
    • GBP antibody
    • HADH antibody
    • Hadhb antibody
    • LCEH antibody
    • LCHAD antibody
    • MGC105338 antibody
    • MGC1728 antibody
    • MGC87480 antibody
    • MS734 antibody
    • MSTP029 antibody
    • MTPA antibody
    • MTPB antibody
    • RGD1560655 antibody
    • TP ALPHA antibody
    • TP-beta antibody
    • Trifunctional enzyme subunit alpha, mitochondrial antibody
    see all

Anti-HADHA/HADHB antibody [4A8BG12] 画像

  • Immunocytochemistry analysis using ab110302 at 5µg/ml staining HADHA/HADHB in HDFn cells.
  • Flow cytometric analysis using ab110302 at 1µg/ml staining HADHA/HADHB in HL60 cells (blue). Isotype control antibody (red).
  • Detection of ab110302 by Western Blot of Immunprecipitate.
    Staining of HADHA/HADHB in 1 mg HepG2 cell lysate/10ul antibody conjugated beads immunoprecipitated using ab110302.
  • Immunohistochemistry analysis using ab110302 staining HADHA/HADHB in Human cerebellum Formalin-fixed, Paraffin-embedded tissue.

Anti-HADHA/HADHB antibody [4A8BG12] (ab110302) 使用論文

ab110302 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Heart)
Permeabilization Yes - Tween-20
Specification Heart
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 29 2016

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Mouse Tissue lysate - whole (Heart)
Specification Heart
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Ms. Arcan Guven

Verified customer

投稿 Sep 03 2014

Thank you very much for your telephone call this morning. As discussed, to our knowledge I am sorry to confirm that ab110302 or ab110281 have not been tested in rabbit samples. Both these antibodies have been raised against the native hum...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"