製品の概要

  • 製品名Anti-HABP2 antibody
    HABP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HABP2
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 288-337 (PTEPSTKLPG FDSCGKTEIA ERKIKRIYGG FKSTAGKHPW QASLQSSLPL) of human HABP2 (NP_004123).

  • ポジティブ・コントロール
    • MCF7 cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab81490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Cleaves the alpha-chain at multiple sites and the beta-chain between 'Lys-53' and 'Lys-54' but not the gamma-chain of fibrinogen and therefore does not initiate the formation of the fibrin clot and does not cause the fibrinolysis directly. It does not cleave (activate) prothrombin and plasminogen but converts the inactive single chain urinary plasminogen activator (pro-urokinase) to the active two chain form. Activates coagulation factor VII.
  • 組織特異性Ubiquitously expressed.
  • 配列類似性Belongs to the peptidase S1 family.
    Contains 3 EGF-like domains.
    Contains 1 kringle domain.
    Contains 1 peptidase S1 domain.
  • 翻訳後修飾Proteolytic cleavage at Gly-23 or Met-27 can give rise to the 50 kDa heavy chain and cleavage at Arg-313 or Lys-319 can give rise to the 27 kDa light chain. The heavy chain can undergo further proteolytic cleavage at Lys-169 or Arg-170 to give rise to 2 inactive 26 kDa fragments and the light chain can undergo further proteolytic cleavage at Arg-480 to give rise to inactive 17 kDa and 8 kDa fragments.
  • 細胞内局在Secreted. Secreted as an inactive single-chain precursor and is then activated to a heterodimeric form.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Factor seven activating protease antibody
    • Factor seven-activating protease antibody
    • Factor VII activating protein antibody
    • Factor VII-activating protease antibody
    • FSAP antibody
    • HABP 2 antibody
    • HABP antibody
    • Habp2 antibody
    • HABP2_HUMAN antibody
    • Hepatocyte growth factor activator like protein antibody
    • Hepatocyte growth factor activator-like protein antibody
    • HGFAL antibody
    • Hyaluronan binding protein 2 antibody
    • Hyaluronan-binding protein 2 27 kDa light chain alternate form antibody
    • Hyaluronic acid binding protein 2 antibody
    • PHBP antibody
    • Plasma hyaluronan binding protein antibody
    • Plasma hyaluronan-binding protein antibody
    see all

Anti-HABP2 antibody 画像

  • Anti-HABP2 antibody (ab81490) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
    Additional bands at : 27 kDa,28 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of HABP2 staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab81490, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-HABP2 antibody (ab81490) 使用論文

This product has been referenced in:
  • Jiang F  et al. Serum Proteome Changes in Healthy Subjects with Different Genotypes of NOS1AP in the Chinese Population. J Diabetes Res 2013:357630 (2013). WB ; Human . Read more (PubMed: 23671866) »

See 1 Publication for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"