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YPYDVPDYAconjugated to KLH, corresponding to amino acids 98-106 of Influenza A Virus Hemagglutinin HA tag
Our Abpromise guarantee covers the use of ab49969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|ICC/IF||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
|ICC||Use at an assay dependent concentration.|
|WB||1/10000. Predicted molecular weight: 63 kDa.|
ab49969 Immunoprecipitating HA tag in HA tagged protein transfected 293T whole cell lysate. 25µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting anti-mouse HRP (ab6789) (1/10000) was used to confirm successful immunoprecipation.
75kD is specific band for HA tagged protein. 120kD, 55kD, 35kD is non specific band for protein A/G bead.