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Synthetic peptide corresponding to HA tag conjugated to Keyhole Limpet Haemocyanin (KLH).
Our Abpromise guarantee covers the use of ab9134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|IHC-Fr||Use at an assay dependent dilution. PubMed: 20551914|
|ELISA||1/100 - 1/500.|
|WB||1/1000 - 1/30000.|
|IP||Use at an assay dependent dilution.|
|ICC||1/200 - 1/2000.|
This image is courtesy of an abreview submitted by Dr Weiguo Zhang.
Immunofluorescence analysis of HeLa R1-11 cells transfected with wtFLAGhPCFTMyc-His10 or FLAGP425RMyc-His10 and wthPCFTHA or wthPCFTHA alone. HA tag was stained with ab9134.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and incubated with primary antibody. An Alexa Fluor® 568-conjugated anti-goat IgG was used as the secondary antibody.