製品の概要

  • 製品名Anti-HA tag antibody
    HA tag 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to HA tag
  • アプリケーション適用あり: WB, ChIP, ICC/IFmore details
  • 種交差性
    Not appplicable.
  • 免疫原

    Synthetic peptide:

    CYPYDVPDYAYPYDVPDYA

    encoding HA tag conjugated to KLH

  • ポジティブ・コントロール
    • HA-tagged recombinant protein bacterial lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度Ammonium Sulphate Precipitation
  • 特記事項(精製)This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab71113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Detects a band of approximately 50 kDa.
ChIP Use at an assay dependent concentration.
ICC/IF 1/10 - 1/50.

ターゲット情報

  • 関連性Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • 別名
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Anti-HA tag antibody 画像

  • All lanes : Anti-HA tag antibody (ab71113)

    Lane 1 : HA-tagged recombinant protein bacterial lysate
    Lane 2 : HA-tagged recombinant protein bacterial lysate

    Lysates/proteins at 12.5 µg per lane.


    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescent staining of MRC-5 fibroblasts transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag using ab71113.

  • ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3-UTR region of GL1 were used in PCR reactions. ACTIN2 was used as a control.

Anti-HA tag antibody (ab71113) 使用論文

This product has been referenced in:
  • Duval N  et al. Msx1 and Msx2 act as essential activators of Atoh1 expression in the murine spinal cord. Development 141:1726-36 (2014). Read more (PubMed: 24715462) »

See 1 Publication for this product

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