製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to HA tag
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 免疫原

    Synthetic peptide within HA tag conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    (Peptide available as ab13835)

  • ポジティブ・コントロール
    • WB: HEK-293 (Human) Whole Cell Lysate - expressing HA tag protein.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab13834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Can be blocked with HA tag peptide (ab13835).

ターゲット情報

  • 関連性
    Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • 別名
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

画像

  • All lanes : Anti-HA tag antibody (ab13834) at 1/500 dilution

    Lane 1 : HEK293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896)
    Lane 2 : HEK293 (Human) Whole Cell Lysate - expressing HA tag protein (ab20896) with HA tag peptide (ab13835) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Alexa Fluor 680 Goat-anti-Rabbit IgG at 1/5000 dilution

    Performed under reducing conditions.

プロトコール

参考文献

This product has been referenced in:
  • Myers SA  et al. SOX2 O-GlcNAcylation alters its protein-protein interactions and genomic occupancy to modulate gene expression in pluripotent cells. Elife 5:e10647 (2016). Read more (PubMed: 26949256) »
  • Chen X  et al. Deficiency or inhibition of lysophosphatidic acid receptor 1 protects against hyperoxia-induced lung injury in neonatal rats. Acta Physiol (Oxf) 216:358-75 (2016). Read more (PubMed: 26495902) »

See all 6 Publications for this product

レビューと Q&A

Application
IHC - Wholemount
Sample
Mouse Tissue (Brain)
Specification
Brain
Username

Prof. Vidita Vaidya

Verified customer

投稿 Jul 23 2014

Thank you for your enquiry, I am pleased to help.

From what I understand here, the customer would liketo detect the HA tag on their recombinant protein expressed in cell culture. This is Immunocytochemistry (ICC). I have reviewed the selecti...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
40 µg
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (10% Bis-Tris)
Blocking step
LI-COR® Odyssey® Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Username

Dr. Svetlana Khoronenkova

Verified customer

投稿 Aug 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human glioma cells)
Loading amount
250000 cells
Specification
Human glioma cells
Treatment
Transfected with lipofectamine
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 04 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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