製品の概要

  • 製品名Anti-HA tag antibody [4C12]
    HA tag 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4C12] to HA tag
  • 特異性The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected cells. The antibody works in ChIP but less well than ab9110 (see ChIP data).
  • アプリケーション適用あり: ELISA, ChIP, ICC, IP, WBmore details

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
  • Concentration information loading...
  • ポリ/モノモノクローナル
  • クローン名4C12
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000. PubMed: 17167031
ChIP Use a concentration of 3 µg/ml. Use 3 µg per 25 µg chromatin (see figure legend).
ICC Use a concentration of 10 µg/ml.
IP Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml.

ターゲット情報

  • 関連性Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • 別名
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Anti-HA tag antibody [4C12] 画像

  • This picture shows baby hamster kidney cell expressing HA-tag CFTR protein. This type of cells were grown on coverslip and immunofluorescence was performed. Dilution of 1:200 was used and incubated for 2hrs with antibody.

  • Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the Abcam X-ChIP protocol. Oocytes were fixed with formaldehyde for 10min. The  ChIP was performed with 25µg of chromatin, 3µg of ab1424 (anti-HA, light blue)  and 3µg of ab18337 (anti-BORIS, dark blue), and 20µl of Protein A/G sepharose beads. A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).

  • Anti-HA tag antibody [4C12] (ab1424) at 1/1000 dilution + HEK293T whole cell lysate over-expressing HA-tagged Rab6 at 20 µg

    Secondary
    HRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 5 seconds

    This image is courtesy of an anonymous Abreview

    Blocked with 5% milk for 30 min at 25°C

    See Abreview

Anti-HA tag antibody [4C12] (ab1424) ��用論文

This product has been referenced in:
  • Graczyk D  et al. Involvement of RNA Polymerase III in Immune Responses. Mol Cell Biol 35:1848-59 (2015). Read more (PubMed: 25776554) »
  • Stanne TM  et al. Identification of the ISWI Chromatin Remodeling Complex of the Early Branching Eukaryote Trypanosoma brucei. J Biol Chem 290:26954-67 (2015). Read more (PubMed: 26378228) »

See all 15 Publications for this product

Product Wall

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Human osteosarcoma)
Total protein in input 1000 µg
Treatment Plasmid transfection for 24 hrs
Immuno-precipitation step Protein A/G
Specification Human osteosarcoma
Username

Miss. Can Zhou

Verified customer

投稿 Jan 28 2016

Application Western blot
Sample Mouse Cell lysate - whole cell (Myoblast)
Gel Running Conditions Non-reduced Denaturing (10% gel)
Loading amount 40 µg
Treatment Plasmid transfection
Specification Myoblast
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Abcam user community

Verified customer

投稿 Jan 28 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (vascular smooth muscle cell)
Permeabilization Yes - NP40
Specification vascular smooth muscle cell
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 06 2016

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Mar 03 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Retro virus transduction for protein X)
Total protein in input 500 µg
Specification Retro virus transduction for protein X
Immuno-precipitation step Protein A/G
Username

Abcam user community

Verified customer

投稿 Feb 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (HEK 293T cells)
Total protein in input 1000 µg
Specification HEK 293T cells
Immuno-precipitation step Protein A
Username

Abcam user community

Verified customer

投稿 Jan 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293T)
Loading amount 20 µg
Specification HEK293T
Gel Running Conditions Reduced Denaturing (12% Tris-glycine gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 17 2012

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