製品の概要

  • 製品名Anti-HA tag antibody [2C16] (Biotin)
    HA tag 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2C16] to HA tag (Biotin)
  • 標識Biotin
  • 特異性This antibody detects HA tags fused to the C terminus or N terminus of targeted proteins in transfected mammalian cells.
  • アプリケーション適用あり: WB, IPmore details
  • 免疫原

    Synthetic peptide - (YPYDVPDYA), derived from the human influenza hemaglutinin protein.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, 1.0% BSA, PBS, pH 7.2
  • Concentration information loading...
  • 特記事項(精製)Dialyzed.
  • ポリ/モノモノクローナル
  • クローン名2C16
  • アイソタイプIgG2b
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab27987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at a concentration of 2 - 10 µg/ml.
    WB: Use at a concentration of 2 - 10 µg/ml.
    Immunostaining techniques of 2 - 10 µg/ml

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
    • 別名
      • HA epitope tag antibody
      • HA1 antibody
      • HA2 antibody
      • hemagglutinin antibody
      • Hemagglutinin HA1 chain antibody
      • Hemagglutinin HA2 chain antibody
      see all

    Anti-HA tag antibody [2C16] (Biotin) (ab27987) 使用論文

    This product has been referenced in:
    • Volná P  et al. Flow cytometric analysis of DNA binding and cleavage by cell surface-displayed homing endonucleases. Nucleic Acids Res 35:2748-58 (2007). Flow Cyt . Read more (PubMed: 17426121) »

    See 1 Publication for this product

    Product Wall

    This antibody has actually been used in the following publication:

    Volná P et al. Flow cytometric analysis of DNA binding and cleavage by cell surface-displayed homing endonucleases. Nucleic Acids Res 35:2748-58 (2007).

    If you...

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    Thank you for your reply.
    If the antibody ab27987 fails to work in either an IP or western blot and we cannot help you identify the cause of this and solve this issue for you, then yes you would be eligible for a refund or replacement.
    You ca...

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    I am sorry that you are disappointed with one of our products. As you have already opened and used the product, we are unable to accept it as a return.
    Under our Abpromise we guarantee our products to work in the applications and species listed on...

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    Thank you for contacting us.
    We unfortunately haven't done any test on the degree of Biotinylation for this antibody. The antibody is however guaranteed to be used in applications as listed on the datasheet. I am afraid we will not be able to prov...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"