Anti-HA tag 抗体 [16B12] (Phycoerythrin) (ab72479)

製品の概要

  • 製品名Anti-HA tag antibody [16B12] (Phycoerythrin)
    HA tag 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [16B12] to HA tag (Phycoerythrin)
  • 標識Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特異性ab72479 recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.
  • アプリケーション適用あり: Flow Cytmore details
  • 免疫原

    Raised against the twelve amino acid peptide CYPYDVPDYASL.

  • エピトープab72479 recognizes the influenza hemagglutinin epitope (YPYDVPDYA).
  • 特記事項

    Excitation max. lambda: 565>498 nm Emission max. lambda: 578 nm.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 2mM Sodium Azide
    Constituents: 0.1% BSA, 150mM Sodium chloride, 100mM Sodium phosphate
  • Concentration information loading...
  • 精製度Size exclusion
  • 特記事項(精製)Size exclusion chromatography as well as proprietary methods for purifying the final materials were used.
  • ポリ/モノモノクローナル
  • クローン名16B12
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • 別名
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Anti-HA tag antibody [16B12] (Phycoerythrin) 画像

  • Flow cytometry analysis of a recombinant (N-terminus HA-tag) transmembrane cell surface receptor in CHO cells using ab72479 (blue). Black - Mouse IgG1 Phycoerythrin isotype control.

Anti-HA tag antibody [16B12] (Phycoerythrin) (ab72479) 使用論文

This product has been referenced in:
  • Yugawa T  et al. Noncanonical NOTCH signaling limits self-renewal of human epithelial and induced pluripotent stem cells through ROCK activation. Mol Cell Biol 33:4434-47 (2013). Read more (PubMed: 24019071) »

See 1 Publication for this product

Product Wall

ab72479:
Like most phycobiliprotein conjugates, the ratio of the fluor to the antibody (F/P) can vary slightly from lot to lot due to the nature of the conjugation chemistry and the methods used to characterize the final product, but it is typicall...

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ab72479 recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus. You should be able to use it for intracellular staining or extracellular staining.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"