Anti-HA tag 抗体 [16B12] (DyLight® 650) (ab117515)

製品の概要

  • 製品名Anti-HA tag antibody [16B12] (DyLight® 650)
    HA tag 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [16B12] to HA tag (DyLight® 650)
  • 標識DyLight® 650. Ex: 654nm, Em: 673nm
  • 特異性ab117515 recognizes the Influenza hemagglutinin epitope (YPYDVPDYA).
  • アプリケーション適用あり: ICC/IF, Flow Cytmore details
  • 免疫原

    Synthetic peptide

    YPYDVPDYA

    corresponding to HA tag.

  • 特記事項

    Stable for 1yr.

    Conjugated to Dylight® 650 (ratio 1.3:): Visible absorption maxima 652 nm; Emission maximum 672 nm.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーpH: 8.20
    Constituent: 99% Tris buffered saline
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名16B12
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab117515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500 - 1/5000.
Flow Cyt Use at an assay dependent concentration.

ターゲット情報

  • 関連性Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
  • 別名
    • HA epitope tag antibody
    • HA1 antibody
    • HA2 antibody
    • hemagglutinin antibody
    • Hemagglutinin HA1 chain antibody
    • Hemagglutinin HA2 chain antibody
    see all

Anti-HA tag antibody [16B12] (DyLight® 650) 画像

  • ab117515 staining 293T cells expressing a HA-tagged protein (intracellular) by Flow Cytometry. Cells were permeabilized and fixed. The sample was incubated with the primary antibody (1/200) for 30 minutes at 4°C.

    Gating Strategy: Gated on FSC-SSC to exclude debris/dead cells.

    Grey - unstained cells.

    Red - Wild type 293T, stained.

    Blue - 293T expressing HA-tagged protein, stained.

    See Abreview

Anti-HA tag antibody [16B12] (DyLight® 650) (ab117515) 使用論文

ab117515 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Fixation BD Fix/Perm
Permeabilization Yes - BD Perm/Wash
Sample Human Cell (293T)
Specification 293T
Gating Strategy Gated on FSC-SSC to exclude debris/dead cells
Preparation Cell harvesting/tissue preparation method: WT and cells expressing an HA-tagged protein (intracellular)
Sample buffer: BD fix/perm and perm/wash
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投稿 Mar 11 2015

We would recommend dilution in TBS as this is the storage buffer the antibody ships with. After dilution we recommend long term storage at -20C. I have attached our Buffers and stock solutions pdf which includes TBS recipes. Your caluculations are c...

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Vielen Dank für das Ausfüllen des Fragenbogens.
Bitte bestätigen Sie mir, dass die Expression des HA-tags auf andere Weise nachgewiesen wurde.
Ich kann Ihnen dann gerne ein neues Röhrchen (auch von einem alternativen Antikörper) als Ersatz sc...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"