製品の概要

  • 製品名
    HA-1077 (Fasudil)
  • 製品の詳細
    ROCK2 inhibitor
  • 別名
    • Fasudil
  • 生理活性の詳細
    ROCK2 inhibitor (IC50 = 1.9 µM). Also shown to inhibit PRK2 (IC50 = 4 µM), MSK1 (IC50 = 5 µM), Rsk2 (IC50 = 15 µM), PKA, p70 S6 Kinase, CaM Kinase and MLCK. Calcium antagonist and vasodilator.

    Also available in simple stock solutions (ab146700) - add 1 ml of water to get an exact, ready-to-use concentration.
  • 精製度
    > 98%

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab120306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

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  • ab46154 staining VEGF in MCF7 cells treated with HA 1077 (ab120306), by ICC/IF. Decrease in VEGF expression correlates with increased concentration of HA 1077 as described in literature.
    The cells were incubated at 37°C for 1h in media containing different concentrations of ab120306 (HA 1077) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab46154 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

参考文献

This product has been referenced in:
  • Bain J  et al. The selectivity of protein kinase inhibitors: a further update. Biochem J 408:297-315 (2007). Read more (PubMed: 17850214) »
  • Davies SP  et al. Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem J 351:95-105 (2000). Read more (PubMed: 10998351) »
  • Amano M  et al. The COOH terminus of Rho-kinase negatively regulates rho-kinase activity. J Biol Chem 274:32418-24 (1999). Read more (PubMed: 10542285) »
  • Asano T & Hidaka H New pharmacological strategies in vascular diseases: a novel intracellular calcium antagonist, HA1077 (AT-877) and cerebral vasospasm. Methods Find Exp Clin Pharmacol 12:443-8 (0). Read more (PubMed: 2087143) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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