Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Human Cell lysate - whole cell (U2OS)
Specification
U2OS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TTBS with 1% BSA
Dilution
1/10000
Secondary antibody
Name
Non-Abcam antibody was used: HRP goat anti-Rabbit, Jackson ImmunoResearch
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL
Bands
Specific: 55 kDa Non-specific: none kDa
Exposure
1 minute(s) and 0 second(s)
Additional data
Additional Notes
siRNA against RAD23A was used to show the specificity of the antibody
different time points after siRNA transfection was checked: 48, 72 and 96hr
non specific siRNA (siLUC) was used as a negative control
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 02 2014