Application
Western blot
Sample
Mouse Cell lysate - whole cell (fibroblast)
Loading amount
50 µg
Specification
fibroblast
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL+
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: nil kDa Non-specific: 80 kDa
Positive control
Hela cell lysate
Additional data
Abcam response
Thank you for your feedback. To our knowledge, this antibody has not been previously tested on mouse lysates. May be the antibody is not suitable for the mouse species however we will wait for more Abreview data to confirm it. We would recommend using more diluted primary and secondary antibodies this may help to detect intact band in mouse lysates.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 10 2013