The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Oxidizes glucose-6-phosphate and glucose, as well as other hexose-6-phosphates.
Present in most tissues examined, strongest in liver.
Defects in H6PD are a cause of cortisone reductase deficiency (CRD) [MIM:604931]. In CRD, activation of cortisone to cortisol does not occur, resulting in adrenocorticotropin-mediated androgen excess and a phenotype resembling polycystic ovary syndrome (PCOS).
In the N-terminal section; belongs to the glucose-6-phosphate dehydrogenase family. In the C-terminal section; belongs to the glucosamine/galactosamine-6-phosphate isomerase family. 6-phosphogluconolactonase subfamily.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling H6PD with ab84353 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of alveolar type I cells. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - H6PD. Right - Merge.
Western blot - H6PD antibody (ab84353)
Anti-H6PD antibody (ab84353) at 1 µg/ml + 721_B cell lysate at 10 µg
Secondary anti-Rabbit IgG HRP at 1/50000 dilution
Predicted band size: 89 kDa Observed band size: 89 kDa Additional bands at: 42 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Ghnenis AB et al. Maternal obesity in the ewe increases cardiac ventricular expression of glucocorticoid receptors, proinflammatory cytokines and fibrosis in adult male offspring. PLoS One12:e0189977 (2017).
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