Anti-Histone H2A.Z (acetyl K7) 抗体 [RM222] (ab214730)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM222] to Histone H2A.Z (acetyl K7)
- Suitable for: ELISA, ICC/IF, WB
- Reacts with: Human
製品の概要
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製品名
Anti-Histone H2A.Z (acetyl K7) antibody [RM222]
Histone H2A.Z 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [RM222] to Histone H2A.Z (acetyl K7) -
由来種
Rabbit -
特異性
ab214730 reacts to Histone H2A.Z acetylated at Lysine 7 (K7ac). No cross reactivity with non-modified Lysine 7 or other acetylated Lysines in histone H2A. -
アプリケーション
適用あり: ELISA, ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: a wide range of other species -
免疫原
Synthetic peptide corresponding to Human Histone H2A.Z (acetyl K7).
Database link: P0C0S5 -
ポジティブ・コントロール
- Acid extracts from HeLa cells treated with sodium butyrate.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
RM222 -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab214730の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use a concentration of 0.2 - 1 µg/ml.
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ICC/IF |
Use a concentration of 1 - 2 µg/ml.
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WB | (3) |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 14 kDa.
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特記事項 |
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ELISA
Use a concentration of 0.2 - 1 µg/ml. |
ICC/IF
Use a concentration of 1 - 2 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 14 kDa. |
ターゲット情報
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機能
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. -
配列類似性
Belongs to the histone H2A family. -
翻訳後修飾
Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
Not phosphorylated. -
細胞内局在
Nucleus. Chromosome. - Information by UniProt
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参照データベース
- Entrez Gene: 3015 Human
- Omim: 142763 Human
- SwissProt: P0C0S5 Human
- Unigene: 119192 Human
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別名
- H2A histone family member Z antibody
- H2A.z antibody
- H2A/z antibody
see all
画像
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All lanes : Anti-Histone H2A.Z (acetyl K7) antibody [RM222] (ab214730) at 0.5 µg/ml
Lane 1 : acid extracts from HeLa cells treated with sodium butyrate
Lane 2 : acid extracts from HeLa cells, untreated
Predicted band size: 14 kDa -
ELISA analysis. ab214730 specifically reacts to Histone H2A.Z acetylated
at Lysine 7 (K7ac). No cross reactivity with non-modified
Lysine 7 or other acetylated Lysines in histone H2A.
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Immunocytochemical staining of HeLa (human epithelial cell line from cervix adenocarcinoma) cells treated with sodium butyrate, stained for Histone H2A.Z (acetyl K7) using ab214730 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab214730 は 2 報の論文で使用されています。
- Feng J et al. Characterization of Cancer Stem Cell Characteristics and Development of a Prognostic Stemness Index Cell-Related Signature in Oral Squamous Cell Carcinoma. Dis Markers 2021:1571421 (2021). PubMed: 34840626
- Wang G et al. Systematic analysis of the lysine acetylome reveals diverse functions of lysine acetylation in the oleaginous yeast Yarrowia lipolytica. AMB Express 7:94 (2017). WB . PubMed: 28497289