製品の概要

  • 製品名Anti-H Cadherin antibody
    H Cadherin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to H Cadherin
  • 特異性This antibody is specific for H Cadherin.
  • アプリケーション適用あり: IP, IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A 15 amino acid peptide from near the amino terminus of human H Cadherin.

  • ポジティブ・コントロール
    • 3T3 cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab36905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use a concentration of 5 µg/ml.
IHC-P 1/1000.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 78 kDa.

ターゲット情報

  • 機能Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth.
  • 組織特異性Highly expressed in heart. In the CNS, expressed in cerebral cortex, medulla, hippocampus, amygdala, thalamus and substantia nigra. No expression detected in cerebellum or spinal cord.
  • 配列類似性Contains 5 cadherin domains.
  • 発生段階Expressed at higher levels in adult brain than in developing brain.
  • 細胞内局在Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CAD13_HUMAN antibody
    • Cadherin-13 antibody
    • CDH13 antibody
    • CDHH antibody
    • H-cadherin antibody
    • Heart cadherin antibody
    • P105 antibody
    • T cad antibody
    • T Cadherin antibody
    • T-cad antibody
    • T-cadherin antibody
    • Truncated cadherin antibody
    see all

Anti-H Cadherin antibody 画像

  • H Cadherin was immunoprecipitated using 0.5mg NIH3T3 whole cell extract, 5µg of Rabbit polyclonal to H Cadherin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, NIH3T3 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab36905.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 78kDa; H Cadherin.

  • Lane 1 : Anti-H Cadherin antibody (ab36905) at 0.5 µg/ml
    Lane 2 : Anti-H Cadherin antibody (ab36905) at 1 µg/ml

    Lane 1 : 3T3 cell lysate.
    Lane 2 : 3T3 cell lysate.


    Predicted band size : 78 kDa
  • ab36905 at 20µg/ml staining H Cadherin in K562 cells by ICC/IF

  • ab36905 staining H Cadherin in human kidney tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in paraformaldehyde and an antigen retrieval step was performed using TRIS-EDTA Buffer pH 9.0. Cells were then permeabilized and incubated with ab36905 at a 1/1000 dilution for 30 minutes at 20°C. The secondary used was an undiluted HRP conjugated goat polyclonal.

    See Abreview

Anti-H Cadherin antibody (ab36905) 使用論文

This product has been referenced in:
  • Wang Q  et al. Down-regulation of adiponectin receptors in osteoarthritic chondrocytes. Cell Biochem Biophys 70:491-7 (2014). Read more (PubMed: 24888493) »
  • Kuphal S  et al. H-cadherin expression reduces invasion of malignant melanoma. Pigment Cell Melanoma Res 22:296-306 (2009). WB, ICC/IF ; Human . Read more (PubMed: 19368692) »

See all 2 Publications for this product

Product Wall


I would recommend the protocol used by the end-user who submitted an Abreview (customer data) for ICC/IF. The link to this Abreview is http://www.abcam.com/H-Cadherin-antibody-ab36905-reviews.html?intabreviewid=10118.
I would also recommend th...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (kidney)
Specification kidney
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer (pH 9.0)
Permeabilization Yes - DAKO Wash-Buffer with Tween
Username

Mr. Rudolf Jung

Verified customer

投稿 Mar 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Stem)
Specification Stem
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Mar 05 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"