製品の概要

  • 製品名Anti-GW182 antibody
    GW182 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GW182
  • アプリケーション適用あり: ICC/IF, IHC-Pmore details
    適用なし: WB
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    A 16 amino acid peptide from near the C terminal residues of human GW182 protein (NM_020847; Q8NDV7).

  • ポジティブ・コントロール
    • Brain lysates.

製品の特性

  • 製品の状態Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab84403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins.
    • 組織特異性Ubiquitous.
    • 配列類似性Belongs to the GW182 family.
      Contains 1 RRM (RNA recognition motif) domain.
    • 細胞内局在Cytoplasm > P-body. Mammalian P-bodies are also known as GW bodies.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CAG repeat protein 26 antibody
      • CAGH26 antibody
      • DKFZp666E117 antibody
      • EDIE antibody
      • EMSY interactor protein antibody
      • FLJ22043 antibody
      • Glycine tryptophan protein of 182 kDa antibody
      • Glycine-tryptophan protein of 182 kDa antibody
      • GW1 antibody
      • GW182 antibody
      • GW182 autoantigen antibody
      • Hypothetical protein DKFZp566M143 antibody
      • KIAA1460 antibody
      • MGC75384 antibody
      • OTTHUMP00000122485 antibody
      • OTTHUMP00000195164 antibody
      • OTTHUMP00000195165 antibody
      • Protein GW1 antibody
      • TNR6A_HUMAN antibody
      • TNRC 6A antibody
      • TNRC6 antibody
      • Tnrc6a antibody
      • Trinucleotide repeat containing 6A antibody
      • Trinucleotide repeat containing gene 6A protein antibody
      • Trinucleotide repeat-containing gene 6A protein antibody
      see all

    Anti-GW182 antibody 画像

    • IHC image of ab84403 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84403, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-GW182 antibody (ab84403) 使用論文

    This product has been referenced in:
    • Aqil M  et al. The HIV-1 Nef protein binds argonaute-2 and functions as a viral suppressor of RNA interference. PLoS One 8:e74472 (2013). Read more (PubMed: 24023945) »
    • Chen S  et al. Ago-2-Mediated Slicer Activity Is Essential for Anti-Flaviviral Efficacy of RNAi. PLoS One 6:e27551 (2011). WB ; Human . Read more (PubMed: 22102908) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.5% triton x100
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 10 2012

    DISCOUNT CODE: [XXXXX] Expiration date: [07/03/2012] I am very pleased to hear you would like to accept our offer and test ab[87541] in [ICC/IF]. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this off...

    Read More

    Thank you for contacting us. We have 3 antibodies available in catalogue ab114857, ab87541 and ab84403. These antibodies are Rabbit polyclonal to GW182. Could you check the datasheets and select the product which is suitable for you. All of the...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - other (Mammary cell)
    Loading amount 50 µg
    Specification Mammary cell
    Treatment TGF beta for 3hrs
    Gel Running Conditions Reduced Denaturing (10)
    Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 11 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"