製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GUCY2C
  • アプリケーション
    適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen: EVRGETYLKG RGNETTYWLT GMKDQKFNLP TPPTVENQQR LQAEFSDMIA NSLQKRQAAG IRSQKPRRVA SYKKGTLEYL QLNTTD, corresponding to amino acids 982-1067 of Human GUCY2C according to P25092.

  • ポジティブ・コントロール
    • Human lateral ventricle tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab122404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin.
  • 配列類似性
    Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
    Contains 1 guanylate cyclase domain.
    Contains 1 protein kinase domain.
  • ドメイン
    The protein kinase domain is predicted to be catalytically inactive.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GC-C antibody
    • Guanylyl cyclase C antibody
    • GUC2C antibody
    • GUC2C_HUMAN antibody
    • GUCY2C antibody
    • Heat stable enterotoxin receptor antibody
    • Heat-stable enterotoxin receptor antibody
    • hSTAR antibody
    • Intestinal guanylate cyclase antibody
    • STA receptor antibody
    see all

画像

  • ab122404, at a 1/550 dilution, staining GUCY2C in neuronal cells of paraffin-embedded Human lateral ventricle tissue by immunohistochemistry.

プロトコール

参考文献

ab122404 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116 and HEK293 cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
HCT116 and HEK293 cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

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投稿 Aug 21 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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