製品の概要
-
製品名Anti-GTPBP4 antibody [EPR3500]
GTPBP4 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EPR3500] to GTPBP4
-
アプリケーション適用あり: WB, IP, IHC-P, Flow Cytmore details
-
種交差性交差種: Human
-
免疫原
A synthetic peptide corresponding to residues in Human GTPBP4.
-
ポジティブ･コントロール
- Human fetal kidney, fetal brain, Human placenta and Human testis tissue lysates; Human kidney tissue.
-
特記事項
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
-
精製度Tissue culture supernatant
-
ポリ/モノモノクローナル
-
クローン名EPR3500
-
アイソタイプIgG
-
研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab92342 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | ||
| IP | ||
| IHC-P | ||
| Flow Cyt |
IHC-P: 1/250 - 1/500. Antigen retrieval is recommended.
IP: 1/50.
WB: 1/2000 - 1/5000. Detects a band of approximately 80 kDa (predicted molecular weight: 74 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
-
機能Involved in the biogenesis of the 60S ribosomal subunit.
-
配列類似性Belongs to the GTP1/OBG family. NOG subfamily.
-
細胞内局在Nucleus > nucleolus.
- Information by UniProt
-
参照データベース
- Entrez Gene: 23560 Human
- SwissProt: Q9BZE4 Human
- Unigene: 215766 Human
-
別名
- Chronic renal failure gene protein antibody
- CRFG antibody
- G protein binding protein CRFG antibody
see all
Anti-GTPBP4 antibody [EPR3500] 画像
-
![Western blot - GTPBP4 antibody [EPR3500] (ab92342)](http://a.abcam.com/ps/datasheet/images/92/ab92342/GTPBP4-Primary-antibodies-ab92342-1.jpg)
Western blot - GTPBP4 antibody [EPR3500] (ab92342)All lanes : Anti-GTPBP4 antibody [EPR3500] (ab92342) at 1/2000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human fetal brain tissue lysate
Lane 3 : Human placenta tissue lysate
Lane 4 : Human testis tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 74 kDa
Observed band size : 80 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GTPBP4 antibody [EPR3500] (ab92342)](http://a.abcam.com/ps/datasheet/images/92/ab92342/GTPBP4-Primary-antibodies-ab92342-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GTPBP4 antibody [EPR3500] (ab92342)ab92342 at 1/250 dilution staining GTPBP4 in Human kidney by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.
プロトコール
Anti-GTPBP4 antibody [EPR3500] (ab92342) 使用論文
ab92342 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"