Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse teratoma (right) tissues labelling GTF3C5 with ab74305 at 1/500 (0.4µg/ml). Detection: DAB.
Western blot - GTF3C5 antibody (ab74305)
All lanes : Anti-GTF3C5 antibody (ab74305) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 60 kDa Observed band size: 60 kDa Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - GTF3C5 antibody (ab74305)
20% of immunoprecipitate obtained from 1mg HeLa whole cell lysate using 3µg ab74305 or a control IgG was loaded in lane 1 and 2 respectively. GTF3C5 was then detected by WB, using ab74305 at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 1 second.