The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Use at 2-5 µg/mg of lysate.
Is unsuitable for WB.
Essential for RNA polymerase III to make a number of small nuclear and cytoplasmic RNAs, including 5S RNA, tRNA, and adenovirus-associated (VA) RNA of both cellular and viral origin. Has histone acetyltransferase activity (HAT) with unique specificity for free and nucleosomal H3. May cooperate with GTF3C5 in facilitating the recruitment of TFIIIB and RNA polymerase through direct interactions with BRF1, POLR3C and POLR3F. May be localized close to the A box.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling GTF3C4 with ab74304 at 1/5000 (0.2µg/ml). Detection: DAB.
Immunoprecipitation - GTF3C4 antibody (ab74304)
Detection of Human GTF3C4 by Immunoprecipitation. 1mg HeLa whole cell lysate loaded in lanes 1-4 with other antibodies that recognise an upstream epitope in lanes 1 and 2, ab74304 at 3 µg in lane 3 and control IgG in lane 4. Image shows immunoprecipitated GTF3C4 detected with post IP WB, loading 20% of IP and using ab74304 at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.