製品の概要

  • 製品名Anti-gtf3c3 antibody
    gtf3c3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to gtf3c3
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide corresponding to a region between residue 475 and 525 of human gtf3c3 (NP_036218.1)

  • ポジティブ・コントロール
    • HeLa whole cell lysate. Mouse NIH3T3 whole Cell Lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab74230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 101 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Involved in RNA polymerase III-mediated transcription. Integral, tightly associated component of the DNA-binding TFIIIC2 subcomplex that directly binds tRNA and virus-associated RNA promoters.
  • 配列類似性Contains 11 TPR repeats.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • General transcription factor 3C polypeptide 3 antibody
    • general transcription factor IIIC, polypeptide 3 (102kD) antibody
    • general transcription factor IIIC, polypeptide 3, 102kD antibody
    • GTF3C3 antibody
    • TF3C-gamma antibody
    • TF3C3_HUMAN antibody
    • TFIIIC 102 kDa subunit antibody
    • TFIIIC gamma antibody
    • TFIIIC102 antibody
    • TFIIIC2 102 antibody
    • TFIIICgamma antibody
    • Transcription factor IIIC 102 kDa subunit antibody
    • transcription factor IIIC gamma subunit antibody
    • Transcription factor IIIC subunit gamma antibody
    • transcription factor IIIC, 102 kD antibody
    see all

Anti-gtf3c3 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling gtf3c3 with ab74230 at 1/500 (0.4µg/ml). Detection: DAB.
  • All lanes : Anti-gtf3c3 antibody (ab74230) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell at 50 µg
    Lane 5 : mouse NIH3T3 cell at 50 µg


    Predicted band size : 101 kDa
    Observed band size : 101 kDa


    Exposure time : 3 minutes
  • Lane 1: HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) with ab74230 used for IP at 3 µg/mg lysate.
    Lane 2: control IgG.
    Subsequent Western blot detection peformed using ab74230 at 1 µg/ml.
    Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-gtf3c3 antibody (ab74230) 使用論文

ab74230 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"