Anti-GTF3C2 抗体 - ChIP Grade (ab89113)

製品の概要

  • 製品名Anti-GTF3C2 antibody - ChIP Grade
    GTF3C2 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to GTF3C2 - ChIP Grade
  • アプリケーション適用あり: ChIP, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length GTF3C2 protein (NP_001030598.1).

  • ポジティブ・コントロール
    • HeLa cell lysate, GTF3C2 transfected 293T cell line lysate and HeLa cells.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab89113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ChIP 1/100.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 101 kDa.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能Required for RNA polymerase III-mediated transcription. Component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. May play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1.
  • 配列類似性Contains 4 WD repeats.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • General transcription factor 3C polypeptide 2 antibody
    • General transcription factor IIIC polypeptide 2 beta 110kDa antibody
    • Gtf3c2 antibody
    • KIAA0011 antibody
    • TF3C beta antibody
    • TF3C-beta antibody
    • TF3C2_HUMAN antibody
    • TFIIIC 110 kDa subunit antibody
    • TFIIIC BETA antibody
    • TFIIIC110 antibody
    • Transcription factor IIIC 110 kDa subunit antibody
    • Transcription factor IIIC subunit beta antibody
    see all

Anti-GTF3C2 antibody - ChIP Grade 画像

  • ChIP analysis using ab89113 binding GTF3C2 in iPS derived from Human neuronal. Cells were cross-linked for 10 minutes with  formaldehyde. Samples were incubated with primary antibody(1/100) for 16 hours at 4°C. Protein binding was detected using real-time PCR.
    Positive control: 7SL RNA gene

    Negative Control: GAPDH coding region.

     

    See Abreview

  • Anti-GTF3C2 antibody - ChIP Grade (ab89113) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 101 kDa
    Observed band size : 101 kDa
  • All lanes : Anti-GTF3C2 antibody - ChIP Grade (ab89113) at 1 µg/ml

    Lane 1 : GTF3C2 transfected 293T cell line lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 101 kDa
    Observed band size : 125 kDa (why is the actual band size different from the predicted?)
  • ab89113, at 10 µg/ml staining GTF3C2 in HeLa cells by Immunofluorescence.

Anti-GTF3C2 antibody - ChIP Grade (ab89113) 使用論文

ab89113 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (Gradientgel 4-12)
Sample Mouse Cell lysate - whole cell (Hela,HEK293, fibroblasts, ES cells)
Specification Hela,HEK293, fibroblasts, ES cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Oct 14 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - whole cell (iPS derived neuronal cells)
Specification iPS derived neuronal cells
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step Real-time PCR
Positive control 7SL RNA gene
Negative control GAPDH coding region
Username

Abcam user community

Verified customer

投稿 Apr 11 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"