製品の概要

  • 製品名
    Anti-GTF3A antibody
    GTF3A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GTF3A
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human GTF3A.

    Immunogen の所有権に関して

    (Peptide available as ab90596.)

  • ポジティブ･コントロール
    • This antibody gave a positive signal in human testis and liver tissue lysates as well as the following whole cell lysates: HeLa; Jurkat; HepG2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 41 kDa).

ターゲット情報

  • 機能
    Interacts with the internal control region (ICR) of approximately 50 bases within the 5S RNA genes, is required for correct transcription of these genes by RNA polymerase III. Also binds the transcribed 5S RNA's. May initiate transcription of the 5S ribosomal RNA gene and maintain the stability of transcription of other genes.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Contains 9 C2H2-type zinc fingers.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • AP 2 antibody
    • AP2 antibody
    • Factor A antibody
    • General transcription factor 3A antibody
    • General transcription factor IIIA antibody
    • GTF 3A antibody
    • GTF3A antibody
    • TF3A_HUMAN antibody
    • TFIIIA antibody
    • Transcription factor 3A antibody
    • Transcription factor IIIA antibody
    see all

画像

  • Western blot - Anti-GTF3A antibody (ab76894)
    Western blot - Anti-GTF3A antibody (ab76894)
    All lanes : Anti-GTF3A antibody (ab76894) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : Human liver tissue lysate - total protein (ab29889)
    Lane 5 : Human testis tissue lysate - total protein (ab30257)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 41 kDa
    Observed band size: 38 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 20 kDa, 45 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute


    This antibody was raised against an immunogen that is predicted to cross react with both isoform 1 (41 kDa) and isoform 2 (38 kDa) of GTF3A. We believe that the band observed at 38 kDa corresponds to isoform 2.

プロトコール

参考文献

ab76894 has not yet been referenced specifically in any publications.

ab76894 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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Western blot abreview for Anti-GTF3A antibody

 Excellent
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Sheep Cell lysate - whole cell (ovine B cell line)
Specification
ovine B cell line
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Feb 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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