製品の概要

  • 製品名Anti-GTF2IRD1 antibody [GTF5I102]
    GTF2IRD1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [GTF5I102] to GTF2IRD1
  • アプリケーション適用あり: WB, ELISA, ICC, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment (internal region).

  • ポジティブ・コントロール
    • whole cell lysate from HeLa cells transfected with GTF2IRD1 expression construct. RIPA lysate of HeLa cells transfected with GTF2IRD1.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab51524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50. Detects a band of approximately 130 kDa (predicted molecular weight: 106 kDa).
ELISA Use at an assay dependent dilution.
ICC 1/10.
IP Use at 10 µg/mg of lysate.

ターゲット情報

  • 機能May be a transcription regulator involved in cell-cycle progression and skeletal muscle differentiation. May repress GTF2I transcriptional functions, by preventing its nuclear residency, or by inhibiting its transcriptional activation. May contribute to slow-twitch fiber type specificity during myogenesis and in regenerating muscles. Binds troponin I slow-muscle fiber enhancer (USE B1). Binds specifically and with high affinity to the EFG sequences derived from the early enhancer of HOXC8.
  • 組織特異性Highly expressed in adult skeletal muscle, heart, fibroblast, bone and fetal tissues. Expressed at lower levels in all other tissues tested.
  • 関連疾患Note=GTF2IRD1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2IRD1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • 配列類似性Belongs to the TFII-I family.
    Contains 5 GTF2I-like repeats.
  • 発生段階Highly expressed in developing and regenerating muscles, at the time of myofiber diversification.
  • ドメインThe N-terminal half may have an activating activity.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CREAM1 antibody
    • General transcription factor II I repeat domain containing protein 1 antibody
    • General transcription factor II-I repeat domain-containing protein 1 antibody
    • General transcription factor III antibody
    • GT2D1_HUMAN antibody
    • GTF2I repeat domain containing protein 1 antibody
    • GTF2I repeat domain-containing protein 1 antibody
    • GTF2IRD1 antibody
    • GTF3 antibody
    • Muscle TFII I repeat domain-containing protein 1 antibody
    • Muscle TFII-I repeat domain-containing protein 1 antibody
    • MUSTRD1 antibody
    • MusTRD1/BEN antibody
    • RBAP2 antibody
    • Slow muscle fiber enhancer binding protein antibody
    • Slow-muscle-fiber enhancer-binding protein antibody
    • USE B1 binding protein antibody
    • USE B1-binding protein antibody
    • WBSCR11 antibody
    • WBSCR12 antibody
    • Williams Beuren syndrome chromosome region 11 protein antibody
    • Williams-Beuren syndrome chromosomal region 11 protein antibody
    • Williams-Beuren syndrome chromosomal region 12 protein antibody
    see all

Anti-GTF2IRD1 antibody [GTF5I102] 画像

  • Anti-GTF2IRD1 antibody [GTF5I102] (ab51524) at 1/50 dilution + Whole cell lysate from HeLa cells transfected with GTF2IRD1 expression construct at 50 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 106 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
  • ab51524 used at 5µg/500µg RIPA lysate of HeLa cells transfected with GTF2IRD1. Western blot was labelled with ab51524 at 1/50 dilution followed by Mouse IgG antibody at 1/2500 dilution. Developed using the ECL technique.
  • HeLa cells stained with ab51524 at 1/10 dilution followed by Alexa Fluor 488 Goat Anti Mouse IgG at 1/200 dilution. Nuclear stain: Hoescht 33342

Anti-GTF2IRD1 antibody [GTF5I102] (ab51524) 使用論文

ab51524 has not yet been referenced specifically in any publications.

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