製品の概要

  • 製品名Anti-GTF2IRD1 antibody
    GTF2IRD1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GTF2IRD1
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: TFGSQNLERI LAVADKIKFT VTRPFQGLIP KPDEDDANRL GEKVILREQV, corresponding to internal sequence amino acids 757-806 of Human GTF2IRD1

  • ポジティブ・コントロール
    • Paraffin embedded Human kidney tissue

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab55975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 106 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能May be a transcription regulator involved in cell-cycle progression and skeletal muscle differentiation. May repress GTF2I transcriptional functions, by preventing its nuclear residency, or by inhibiting its transcriptional activation. May contribute to slow-twitch fiber type specificity during myogenesis and in regenerating muscles. Binds troponin I slow-muscle fiber enhancer (USE B1). Binds specifically and with high affinity to the EFG sequences derived from the early enhancer of HOXC8.
  • 組織特異性Highly expressed in adult skeletal muscle, heart, fibroblast, bone and fetal tissues. Expressed at lower levels in all other tissues tested.
  • 関連疾患Note=GTF2IRD1 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2IRD1 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.
  • 配列類似性Belongs to the TFII-I family.
    Contains 5 GTF2I-like repeats.
  • 発生段階Highly expressed in developing and regenerating muscles, at the time of myofiber diversification.
  • ドメインThe N-terminal half may have an activating activity.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CREAM1 antibody
    • General transcription factor II I repeat domain containing protein 1 antibody
    • General transcription factor II-I repeat domain-containing protein 1 antibody
    • General transcription factor III antibody
    • GT2D1_HUMAN antibody
    • GTF2I repeat domain containing protein 1 antibody
    • GTF2I repeat domain-containing protein 1 antibody
    • GTF2IRD1 antibody
    • GTF3 antibody
    • Muscle TFII I repeat domain-containing protein 1 antibody
    • Muscle TFII-I repeat domain-containing protein 1 antibody
    • MUSTRD1 antibody
    • MusTRD1/BEN antibody
    • RBAP2 antibody
    • Slow muscle fiber enhancer binding protein antibody
    • Slow-muscle-fiber enhancer-binding protein antibody
    • USE B1 binding protein antibody
    • USE B1-binding protein antibody
    • WBSCR11 antibody
    • WBSCR12 antibody
    • Williams Beuren syndrome chromosome region 11 protein antibody
    • Williams-Beuren syndrome chromosomal region 11 protein antibody
    • Williams-Beuren syndrome chromosomal region 12 protein antibody
    see all

Anti-GTF2IRD1 antibody 画像

  • Anti-GTF2IRD1 antibody (ab55975) at 2.5 µg/ml + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 106 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 8%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling GTF2IRD1 with ab55975 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

Anti-GTF2IRD1 antibody (ab55975) 使用論文

ab55975 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblast)
Loading amount 25 µg
Specification Mouse Embryonic Fibroblast
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Feb 18 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"