製品の概要

  • 製品名Anti-GTF2H1 antibody
    GTF2H1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to GTF2H1
  • アプリケーション適用あり: WB, ICC/IF, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-549 of Human GTF2H1

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
Flow Cyt Use 0.01-0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.
  • 配列類似性Contains 2 BSD domains.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Basic transcription factor 2 62 kDa subunit antibody
    • Basic transcription factor 62 kDa subunit antibody
    • BTF 2 antibody
    • BTF2 antibody
    • BTF2 p62 antibody
    • General transcription factor IIH polypeptide 1 antibody
    • General transcription factor IIH subunit 1 antibody
    • General transcription factor IIH, polypeptide 1, 62kDa antibody
    • gtf2h1 antibody
    • TF2H1 antibody
    • TF2H1_HUMAN antibody
    • TFB1 antibody
    • TFIIH antibody
    • TFIIH basal transcription factor complex p62 subunit antibody
    see all

Anti-GTF2H1 antibody 画像

  • Overlay histogram showing HeLa cells stained with ab55199 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55199, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • GTF2H1 antibody (ab55199) used in immunofluorescence at 1-5ug/ml on HeLa cells.
  • GTF2H1 antibody (ab55199) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human testis tissue.
  • GTF2H1 antibody (ab55199) at 1ug/lane + Jurkat cell lysate at 25ug/lane.

Anti-GTF2H1 antibody (ab55199) 使用論文

This product has been referenced in:
  • Kainulainen M  et al. Virulence factor NSs of rift valley fever virus recruits the F-box protein FBXO3 to degrade subunit p62 of general transcription factor TFIIH. J Virol 88:3464-73 (2014). WB . Read more (PubMed: 24403578) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (testes)
Specification testes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Mar 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"