製品の概要

  • 製品名
    Anti-GSTM1 antibody
    GSTM1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GSTM1
  • 由来種
    Rabbit
  • 特異性
    Ab77925 is specific for GSTM1 but there is a possibility of cross-reactivity with other isoforms of GST.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    C-EKLKPKYLEELPEK

    , corresponding to amino acids 121-134 of Mouse GST M1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • Hela cell lysate. Liver.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: Use at a concentration of 0.5 - 2.0 µg/ml for 2 hours at RT. Detects a band of approximately 26 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • ターゲット情報

    画像

    • Western blot - Anti-GSTM1 antibody (ab77925)
      Western blot - Anti-GSTM1 antibody (ab77925)
      Anti-GSTM1 antibody (ab77925) at 0.5 µg/ml + Hela cell lysate

      Secondary
      Goat anti-Rabbit HRP conjugated at 1/2000 dilution

      Developed using the ECL technique.

      Observed band size: 26 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.

    プロトコール

    参考文献

    This product has been referenced in:
    • Sadi G  et al. Differential gene expression in liver tissues of streptozotocin-induced diabetic rats in response to resveratrol treatment. PLoS One 10:e0124968 (2015). WB ; Rat . Read more (PubMed: 25905778) »
    • Hunter A  et al. DNA Methylation Is Associated with Altered Gene Expression in AMD. Invest Ophthalmol Vis Sci 53:2089-105 (2012). WB, ICC/IF ; Human . Read more (PubMed: 22410570) »

    See all 2 Publications for this product

    ab77925 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    1 Review or Q&A

    10
    Votes: Highest First

    I'm looking for a non-conjugated primary antibody that is specific for GSTM1. I have looked at your website and I'm not sure if they have high cross-reactivity with the other mu isoforms.



    I can confirm that none of our antibodies below against GSTM1 has been experimentally tested for its cross-reactivity with other family member.

    I have therefore checked for homology with the immunogen:


    ab77925:
    ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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