製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GSTA2
  • 特異性
    The immunogen of ab102760 shares 86.7% sequence homology with P08263 GSTA1_HUMAN. For this reason this antibody will probably detect GSTA1. However we haven't performed any cross-reactivity assays with other GSTAs.
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    KLH conjugated synthetic peptide corresponding to a region within N terminal amino acids 1-30 of Human GSTA2.

  • ポジティブ・コントロール
    • MDA-MB231 cell lysate; Human hepatocarcinoma

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab102760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 26 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 関連性
    Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, which are located in a cluster on chromosome 6, are highly related and encode enzymes with glutathione peroxidase activity that function in the detoxification of lipid peroxidation products. Reactive electrophiles produced by oxidative metabolism have been linked to a number of degenerative diseases including Parkinson's disease, Alzheimer's disease, cataract formation, and atherosclerosis.
  • 細胞内局在
    Cytoplasmic
  • 参照データベース
  • 別名
    • EC 2.5.1.18 antibody
    • Glutathione S transferase 2 antibody
    • Glutathione S transferase A2 antibody
    • Glutathione S-alkyltransferase A2 antibody
    • Glutathione S-aralkyltransferase A2 antibody
    • Glutathione S-aryltransferase A2 antibody
    • Glutathione S-transferase alpha 2 antibody
    • Glutathione S-transferase GT41A antibody
    • Glutathione S-transferase Ya-2 antibody
    • Glutathione S-transferase, alpha 2 (Yc2) antibody
    • GST 1b-1b antibody
    • GST A2-2 antibody
    • GST class alpha member 2 antibody
    • GST gamma antibody
    • GST HA subunit 2 antibody
    • GST Ya2 antibody
    • GST, class alpha, 2 antibody
    • GST2 antibody
    • Gst2-2 antibody
    • GSTA2 2 antibody
    • GSTA2-2 antibody
    • Gstc-2 antibody
    • Gstc2 antibody
    • GTA2 antibody
    • GTH2 antibody
    • HA subunit 2 antibody
    • Liver GST2 antibody
    • MGC10525 antibody
    • OTTHUMP00000017883 antibody
    • S-(hydroxyalkyl)glutathione lyase A2 antibody
    see all

画像

  • Anti-GSTA2 antibody (ab102760) at 1/100 dilution + MDA-MB231 cell lysate at 35 µg

    Predicted band size : 26 kDa
  • ab102760, at 1/50 dilution, staining GSTA2 in formalin-fixed, paraffin-embedded Human hepatocarcinoma using a peroxidase-conjugated secondary antibody and DAB staining.

参考文献

ab102760 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rabbit Cell (HEK293 human cells)
Permeabilization
Yes - triton
Specification
HEK293 human cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Formaldehyde
Username

Miss. zohar pode

Verified customer

投稿 Mar 25 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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