製品の概要

  • 製品名Anti-GST3 / GST pi antibody
    GST3 / GST pi 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to GST3 / GST pi
  • 特異性ab53943 is specific for GST pi.
  • アプリケーション適用あり: WB, IHC-P, IP, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Hamster, Human
  • 免疫原

    Full length native protein (purified) GST3/ GST pi (Rat)

  • ポジティブ・コントロール
    • This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HCT116.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/3000. Detects a band of approximately 31 kDa (predicted molecular weight: 23 kDa).
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at an assay dependent concentration. PubMed: 23741294
ICC/IF 1/200.

ターゲット情報

  • 機能Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
  • 配列類似性Belongs to the GST superfamily. Pi family.
    Contains 1 GST C-terminal domain.
    Contains 1 GST N-terminal domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Deafness antibody
    • Deafness X-linked 7 antibody
    • DFN7 antibody
    • FAEES3 antibody
    • Fatty Acid Ethyl Ester Synthase III antibody
    • Glutathione S Transferase 3 antibody
    • Glutathione S Transferase Pi antibody
    • Glutathione S-transferase P antibody
    • Glutathione S-transferase pi 1 antibody
    • GST class-pi antibody
    • GST3 antibody
    • GSTP antibody
    • Gstp1 antibody
    • GSTP1-1 antibody
    • GSTP1_HUMAN antibody
    • PI antibody
    • X linked 7 antibody
    see all

Anti-GST3 / GST pi antibody 画像

  • IHC image of ab53943 staining in human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab53943, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.



  • Predicted band size : 23 kDa
  • ab53943 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab53943 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GST3 / GST pi antibody (ab53943) 使用論文

This product has been referenced in:
  • Klaus A  et al. Glutathione S-Transferases Interact with AMP-Activated Protein Kinase: Evidence for S-Glutathionylation and Activation In Vitro. PLoS One 8:e62497 (2013). IP ; Rat . Read more (PubMed: 23741294) »

See 1 Publication for this product

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (brain)
Loading amount 5 µg
Specification brain
Gel Running Conditions Reduced Denaturing (12% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

投稿 Dec 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Glioblastoma cell line)
Loading amount 20 µg
Specification Glioblastoma cell line
Treatment Lysis buffer (urea/thiourea)
Gel Running Conditions Reduced Denaturing (4-20% 1mm)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Feb 05 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"