製品の概要

  • 製品名
    Anti-GST3 / GST pi antibody
    GST3 / GST pi 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to GST3 / GST pi
  • 由来種
    Goat
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog
  • 免疫原

    Synthetic peptide:

    LADQGQSWKEEV

    , corresponding to Internal sequence amino acids 21-32 of Human GST3

  • ポジティブ・コントロール
    • Human kidney lysates. Lysate prepared from transfected HEK293 cells transiently expressing GST3 / GST pi.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab36149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).

ターゲット情報

画像

  • Anti-GST3 / GST pi antibody (ab36149) at 0.01 µg/ml + Human Kidney lysate (35µg protein in RIPA buffer).

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Primary incubation was 1 hour. Detected by chemiluminescence.
  • Anti-GST3 / GST pi antibody (ab36149) at 0.03 µg/ml + lysate prepared from transfected HEK293 cells transiently expressing GST3 / GST pi

    Predicted band size: 23 kDa
    Observed band size: 26 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

This product has been referenced in:
  • Cooper TK  et al. Use of urinary biomarkers of renal ischemia in a lamb preclinical left ventricular assist device model. Artif Organs 36:820-4 (2012). Sheep . Read more (PubMed: 22428807) »

See 1 Publication for this product

レビューと Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (Eye retinal protein)
Loading amount
50 µg
Specification
Eye retinal protein
Gel Running Conditions
Reduced Denaturing (4-14%)
Blocking step
BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

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Verified customer

投稿 Dec 30 2011

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Thank you for your enquiry. I am sorry I did not mention this earlier but I can offer you a discount of 15% off the order of ab4606, normal mouse kidney slides: http://www.abcam.com/Kidney-mouse-normal-tissue-slides-ab4606.html Include this discount co...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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