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Synthetic peptide mapping to the C terminus of Human Glutathione S Transferase pi, differing from the related Mouse sequence by two amino acids.
Our Abpromise guarantee covers the use of ab106268 in the following tested applications.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 23 kDa.|
|IHC-P||Use a concentration of 1 - 2 µg/ml. Boiling the sections is required.|
|ICC||Use a concentration of 0.5 - 1 µg/ml. In acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required.|
ICC/IF image of ab106268 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab106268 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab106268 has not yet been referenced specifically in any publications.