This antibody was prepared from monospecific antiserum by immunoaffinity
chromatography using GST coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use at an assay dependent dilution.
IM: Use at an assay dependent dilution.
This antibody is also suitable for fluorescent assays requiring lot to lot consistency.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.