製品の概要

  • 製品名Anti-GST antibody
    GST 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GST
  • アプリケーション適用あり: ELISA, IP, WB, ICC/IFmore details
  • 種交差性
    Reacts with Schistosoma japonicum. Not yet tested on other species.
  • 免疫原

    Rabbits were immunized with Glutathione-S-transferase (GST) from Schistosoma japonicum.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab9085 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
IP Use at an assay dependent dilution.
WB 1/500.
ICC/IF Use at an assay dependent dilution. PubMed: 21242312

ターゲット情報

  • 関連性GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
  • 別名
    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

Anti-GST antibody 画像

  • ab9085 staining GST in wild-type (GST–NUAK2-wt) expressing HeLa cells by Immunocytochemistry/ Immunofluorescence.
    Cells were treated with 10µM Y27632 inhibitor for 30 minutes before fixation.
  • All lanes : Anti-GST antibody (ab9085) at 1/2000 dilution

    Lane 1 : Recombinant GST protein purified from E. coli XL1-Blue
    Lane 2 : Recombinant GST-tagged p53 protein purified from E. coli XL1-Blue

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP-conjugated Goat anti-Rabbit IgG polyclonal at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 26,80 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-GST antibody (ab9085) 使用論文

This product has been referenced in:

See all 24 Publications for this product

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (E. coli-purified GST)
Loading amount 0.05 µg
Specification E. coli-purified GST
Gel Running Conditions Reduced Denaturing (10% Acrylamide)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 21 2013

Thank you for contacting us.

While it is likely that this product will work in IF, we have not yet tested ab6613 in this application and therefore cannot guarantee that it will give the desired results in this application. We do have a num...

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Our product team has created this form which I have attached. This should be live on the website as well. Please let me know if you have any other questions or if there is anything else Abcam might do for you.

Thank you for contacting us.

I have contacted our products team and they are currently creating an MSDS for this product. This should be on the website soon. I will send you a copy when this has been created as well.

I hope th...

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Purified protein (Recombinant protein purified from E. coli XL1-Blue)
Loading amount 10 µg
Specification Recombinant protein purified from E. coli XL1-Blue
Gel Running Conditions Reduced Denaturing (12 % PAGE gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 08 2012

I am sorry for the delay in replying to you. I finally got a confirmation from the originator of the product. According to them, as far as they know, this product will detect all forms of GST. If there is anything else that I can help you with,...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"