製品の概要

  • 製品名Anti-GST antibody
    GST 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GST
  • アプリケーション適用あり: ELISA, WB, ICC, IP, ChIPmore details
  • 種交差性
    Schistosoma japonicum
  • 免疫原

    Glutathione-S-Transferase (GST) from Schistosoma japonicum.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab19256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/100 - 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 24 kDa.
ICC 1/100 - 1/400.
IP Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.

ターゲット情報

  • 関連性GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
  • 別名
    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

Anti-GST antibody 画像

  • All lanes : Anti-GST antibody (ab19256) at 1/15000 dilution

    Lane 1 : HEK293 lysate at 0.1 µg
    Lane 2 : HEK293 lysate at 0.3 µg
    Lane 3 : HEK293 lysate at 1 µg


    Predicted band size : 24 kDa


    Exposure time : 3 seconds

    Detected by Chemiluminescence. 

     

  • All lanes : Anti-GST antibody (ab19256) at 1/5000 dilution

    Lane 1 : GST tagged purified protein from E. Coli BL21 cells at 0.1 µg
    Lane 2 : GST tagged purified protein from E. Coli BL21 cells at 0.2 µg
    Lane 3 : GST tagged purified protein from E. Coli BL21 cells at 0.4 µg

    Secondary
    A HRP conjugated donkey polyclonal to rabbit at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 72 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds

    This image is courtesy of an Abreview submitted by Dr Hanneke Okkenhaug

    See Abreview

  • NogoΔ20-induced Rho activation depends on internalization. (A–C) Rho activation levels were examined in PC12 cells that were either untreated (control; A), treated with 300 nM NogoΔ20 for 30 min at 37°C in absence of mutant PincherG68E (B), or treated with 300 nm NogoΔ20 treated for 30 min at 37°C in the presence of mutant PincherG68E (C). Active GTP-bound Rho was detected by incubation with GST-tagged Rhotekin-RBD and immunostaining using ab19256. Bar, 20 µm. (D) Densitometric quantification of staining from three independent experiments. Data are normalized to the mean of the untreated group ± SEM (error bars); asterisks marks highly significant differences between untreated, NogoΔ20-treated, or NogoΔ20- and dn PincherG68E-treated cells (three experiments; 30–50 cells per experiment; ***, P < 0.001; Student’s t test). (E) PC12 cells were either left untreated or transfected with dn PincherG68E construct. All cells were then incubated with 300 nM NogoΔ20 for 30 min at 37°C. Extracted

Anti-GST antibody (ab19256) 使用論文

This product has been referenced in:
  • Chanduri M  et al. Inositol hexakisphosphate kinase 1 (IP6K1) activity is required for cytoplasmic dynein-driven transport. Biochem J 473:3031-47 (2016). Read more (PubMed: 27474409) »
  • Pettit MW  et al. Construction and physiochemical characterisation of a multi-composite, potential oral vaccine delivery system (VDS). Int J Pharm 468:264-71 (2014). WB . Read more (PubMed: 24680960) »

See all 8 Publications for this product

Product Wall

Abreviews
Application Western blot
Loading amount 1 µg
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Sample Mouse Recombinant protein (made in E.coli)
Specification made in E.coli
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投��� Nov 18 2014

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Detection step Semiquantitative PCR
Sample Human Cell lysate - nuclear (MCF7)
Specification MCF7
Negative control IgG USED IMMUNOPRECIPITATED SAMPLE
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control INPUT
Username

Abcam user community

Verified customer

投稿 May 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (mcf7)
Total protein in input 1 µg
Specification mcf7
Immuno-precipitation step Other - sepharose beads
Username

Abcam user community

Verified customer

投稿 Apr 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (mcf 7)
Loading amount 100 µg
Specification mcf 7
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

投稿 Apr 29 2013

Thank you for contacting us.
The ability for an antibody to recognize an epitope tag can be dependent on the recombinant protein and expression system. We do not have experience using this antibody to detect GST dimer. As shown in the datasheet, t...

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Thank you for your call today, and I am so sorry that this was not taken care of yesterday. As we discussed, I am sending a free of charge vial of ab19256 on the order ***, which should arrive tomorrow by 10:30am. The FedEx tracking number for this shi...

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Purified protein (Recombinant GST protein purified from E. coli)
Loading amount 5 µg
Specification Recombinant GST protein purified from E. coli
Gel Running Conditions Reduced Denaturing (10% Tris-Bicine)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jul 30 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Recombinant protein (Western blot to analyse GST pulldown)
Loading amount 15 µg
Specification Western blot to analyse GST pulldown
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 May 14 2010

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Saccharomyces cerevisiae Cell lysate - whole cell (Whole cell)
Specification Whole cell
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: High quality formaldehyde
Detection step Semiquantitative PCR
Username

Azharul Islam

Verified customer

投稿 Feb 02 2009

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Purified protein (GST-tagged protein expressed in BL21* cells, fract)
Loading amount 0.1 µg
Specification GST-tagged protein expressed in BL21* cells, fract
Gel Running Conditions Reduced Denaturing (8% SDS page gel)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Hanneke Okkenhaug

Verified customer

投稿 Nov 05 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"