製品の概要

  • 製品名Anti-GST antibody [3G10/1B3]
    GST 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3G10/1B3] to GST
  • 特異性Recognizes Glutathione S-Transferase (GST), a 26-kDa protein encoded by the parasitic helminth Schistosoma japonicum.
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Species independent
  • 免疫原

    Recombinant full length protein within Schistosoma japonicum GST aa 1-218. The exact sequence is proprietary. This is full length GST fusion protein of 425 aa (length of the fusion protein, not GST protein alone). The GST protein is the same GST protein on the pGEX vector ( Schistosoma japonicum).
    Database link: P08515

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
IP Use at an assay dependent dilution.

ターゲット情報

  • 関連性GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
  • 別名
    • Glutathione S Transferase antibody
    • Glutathione S-transferase class-mu 26 kDa isozyme antibody
    • GST antibody
    • Sj26 antigen antibody
    • SjGST antibody
    see all

Anti-GST antibody [3G10/1B3] 画像

  • All lanes : Anti-GST antibody [3G10/1B3] (ab92) at 1/5000 dilution

    Lane 1 : Non-induced GST-fusion protein expressed in E.coli
    Lane 2 : IPTG induced GST-fusion protein expressed in E.coli

    Lysates/proteins at 5 µl per lane.


    Predicted band size : 26 kDa

    10% SDS-PAGE

  • All lanes : Anti-GST antibody [3G10/1B3] (ab92)

    Lane 1 : Total lysate
    Lane 2 : Cytoplasmic fraction
    Lane 3 : Membrane fraction
    Lane 4 : Nuclear fraction


    Predicted band size : 26 kDa
    Detection of human GST in cytoplasmic fraction by anti-GST 3G10 monoclonal antibody (ab92) in western blot experiment. p84 is also shown indicating the nuclear fraction using anti-p84 monoclonal antibody (ab487).

Anti-GST antibody [3G10/1B3] (ab92) 使用論文

This product has been referenced in:
  • Rossi M  et al. High throughput screening for inhibitors of the HECT ubiquitin E3 ligase ITCH identifies antidepressant drugs as regulators of autophagy. Cell Death Dis 5:e1203 (2014). WB . Read more (PubMed: 24787015) »
  • Mikula M  et al. Heterogeneous nuclear ribonucleoprotein (HnRNP) K genome-wide binding survey reveals its role in regulating 3'-end RNA processing and transcription termination at the early growth response 1 (EGR1) gene through XRN2 exonuclease. J Biol Chem 288:24788-98 (2013). WB . Read more (PubMed: 23857582) »

See all 8 Publications for this product

Product Wall

Thank you for your enquiry and your interest in our products.

I can confirm that we sell this antibody in liquid format, the unit size is 100 µg the concentration is 1 mg/ml.

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I am very glad to hear that our anti-Beta Actin loading control has worked so well for you.

In regards to a loading control for your current needs, Beta Actin would only be present in serum if shearing of cells had occurred during collection...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Lysate from Insect Cells Expressing RGS9-GST fusio)
Loading amount 100 µg
Specification Lysate from Insect Cells Expressing RGS9-GST fusio
Gel Running Conditions Reduced Denaturing (10% Tris Glycine)
Blocking step 1:1 PBS/Licor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 22°C
Username

Dr. Chris Tracy

Verified customer

投稿 Sep 20 2011

I'm sorry to hear you are experiencing problems with ab92. I have been able to source the images from the laboratory and have added them to the datasheet, interestingly the band we get with a HeLa whole cell lysate is also large ("fat") in a simila...

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Thanks again for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should yo...

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Thank you for enquiry. I have checked with the originator of this antibody and there was a mistake regarding the immunogen on the datasheet. This antibody was generated as a bi-product during a fusion. The original immunogen was a GST fusion protei...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"