製品の概要

  • 製品名
    GST Activity Assay Kit (Fluorometric)
    GST キット 製品一覧
  • サンプルの種類
    Saliva, Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Purified protein, Tissue Lysate
  • アッセイタイプ
    Enzyme activity
  • 検出感度
    < 0.5 mU/well
  • 全工程の試験時間
    2h 00m
  • 製品の概要

    Abcam's GST Activity Assay Kit (Fluorometric) provides a simple, fluorescence-based in vitro assay for detecting the GST activity using fluorescence plate reader. The assay utilizes monochlorobimane (MCB), a dye that reacts with glutathione. The free form of MCB is almost nonfluorescent, whereas the dye fluoresces in blue (Ex/Em = 380/461 nm) after reaction with glutathione catalysed by GST. The change in fluorescence overtime allows for an easy measurement of the sample GST level.

    The kit can detect GST activity in crude cell lysate or purified protein fraction, and also quantitate GST-tagged fusion protein.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Glutathione S-transferase (GST) is a family of enzymes that play an important role in detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobiotics. It utilizes glutathione to scavenge potentially toxic compounds including those produced as a result of oxidative stress and is part of the defense mechanism against the mutagenic, carcinogenic and toxic effects of such compounds.

  • アプリケーション
    適用あり: Functional Studiesmore details

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab65325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

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  • Standard Calibration Curve of GST Measured by Fluorometry. Various amounts of standard GST were incubated with GSH and MCB according to the kit instructions. Fluorescence was measured at ex/em 380/460 nm.

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参考文献

ab65325 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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